古方生脉散制剂中人参皂甙的反相高效液相色谱分离和含量测定  

SEPARTION AND QUANTITATIVE DETERMINATION OF GINSENOSIDES IN SHENGMAISAN PREPARATION BY RP-HPLC

作　　者：马鹏[1] 林涛[1] 黄静[1] 

机构地区：[1]华西医科大学药学院

出　　处：《华西药学杂志》1995年第1期21-24,共4页West China Journal of Pharmaceutical Sciences

摘　　要：用反相高效液相色谱法对生脉散制剂中人参皂甙类成分进行分离，并测定处方中人参皂甙Ｒｇｌ含量。用ＩｎｅｒｔｓｉｌＯＤＳ－２柱，乙腈－０．０５％磷酸（１９：８１）为流动相，２０３ｎｍ为检测波长。方法准确、精密、灵敏、分离良好，可用于多种样品分析。In this paper we sueccssfully estabalished a RP-HPLC method for the separation of Ginsenosides in Shengmai San preparation and quantitatively determined Ginsenoside Rgl in preparation,The HPLC conditions were Inertsil ODS-2 column,CH3CN-0.05%H3PO4(19: 81)as mobile phase,detection at 203 nm.The curve of Y=170446.8x+23864.1,r=0.9999 within 25100ng～200.8ng,method recovery was 98.63%,CV%=2.4%(n=9),within day precision CV%=0.21%,Inter day precision CV%=0.48%(n=9),detective limit 100ng (S/N=5:1),lowest detective concentration 10ug/ml(S/N=5:1).The established method was proved to be accurate, precise,sensitive and had a very good separation. It can be empolye to the analysis of various samples.

关 键 词：生脉散制剂 人参皂甙 分析方法 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]