小菜粉蝶胚胎细胞的传代培养  

CONTINUOUS CULTURE OF EMBRYOS CELLS FROM PIERIS RAPAE

作　　者：黎路林 陈曲候 

出　　处：《华中师范大学学报（自然科学版）》1995年第3期364-366,共3页Journal of Central China Normal University：Natural Sciences

摘　　要：小菜粉蝶孵育卵经充分剪碎后，其组织块置ＴＣ－１００培养基中培养７个月后，细胞长成单层，经历第一次传代．由原始细胞培养物传代后分化产生３个细胞大小、形态和生长特征各不相同的细胞系统．A primary culture was set up from of Pieris rapae in TC-100 medium. It was refed 6 mohiths post explanation, and the cells propagated from the tissues culture were subcultured two month later. Three substrains, designated Pr-E (A),Pr-E(B)and Pr-E(C) respectively, developed from the primary cell line. They differ in morphology, behavior and growth of cells. The cells of Pr-E(A),Pr-E(B)and Pr-E(C)in their second, fifth and tenth generation in respect, were preserved at 4℃ for 5 months. The Pr-E(A) and Pr-E(B) cells, which adhere to the bottom of the flasks and grow slowly in culture,recovered after being transfered to incubator at 28℃, But the Pr-E (C) cells, which suspend in medium and grow quickly died during the period.

关 键 词：细胞培养 细胞系 胚胎细胞 传代培养 小菜粉蝶 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治] Q969.438.3[农业科学—植物保护]