α-干扰素的液相色谱/毛细管电泳两维分离分析  

Two Dimensional Separation and Analysis of α-Interfere by LC/ CE

作　　者：蒋银土[1] 杨国梁[1] 商振华[2] 郭为[2] 林炳承[2] 

机构地区：[1]杭州大学化学系,杭州310028 [2]中国科学院大连化学物理研究所,大连116012

出　　处：《化学学报》1995年第4期392-396,共5页Acta Chimica Sinica

基　　金：国家自然科学基金资助的项目.

摘　　要：用亲和色谱/反相色谱、亲和色谱/毛细管电泳和凝胶色谱/毛细管电泳等两维系统,对白细胞提取液中的α-干扰素进行分离分析,并对结果进行比较,充分肯定了亲和色谱/毛细管电泳联用在蛋白质类药物制备纯化中的显著作用。Several two dimensional systems, such as affinity chromatography / reversed phase liquid chromatography, affinity chromatography/capillary electrophoresis and gel permeation chromatography / capillary electrophoresis were used to separate and analysis the α-interfere of the white blood corpuscle extract. The results show that HPLC / CE system plays an important role in purification and preparation.

关 键 词：亲和色谱 毛细管电泳 干扰素 药物纯化 

分 类 号：Q511[生物学—生物化学] O657.8[理学—分析化学]