急性心肌梗塞病人血清巯基蛋白酶抑制肽C的变化  

Changes of Serum Cystatin C in Patients with Acute Myocardial Infarction

作　　者：冯建芳[1] 汪淑荣[1] 李平[1] 赵一鸣[1] 孙诠[1] 师树古[1] 陈明哲[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京医科大学第三医院

出　　处：《基础医学与临床》1995年第4期45-47,共3页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：采用兔抗人ＣｙｓｔａｔｉｎＣ抗体建立了酶联免疫吸附测定（ＥＬＩＳＡ）技术，对急性心肌梗塞病人急性发作期、恢复期及正常对照进行了测定。发现心梗急性发作期和恢复期血清ｃｙｓｔａｔｉｎＣ含量无明显变化，但均明显低于对照（Ｐ＜０．０ｌ）。用Ｌｏｇｉｓｔｉｃ回归模型校正年龄因素的影响后，上述差别仍然显著（Ｐ＝０．０４１）。提示血清ｃｙｓｔａｔｉｎＣ浓度变化在一定程度上可作为急性心肌梗塞诊断的参考指标。此检测方法特异性强、灵敏度高、重复性好。n the present study,an enzyme-linked immunosorbent assay(ELISA )was established byutilizing rabbit anti-human cystatin C antibodies. The levels of serum cystatin C in AMI patientsand control were measured. Further we compared the serum cystatin C concentrations of duringonset with that of convalescence in AMI patients, The results showed that the assay procedurewas highly specific,sensitive and reproducible. The level of serum cystatin C in AMI patients waslower than that in control(P<0. 0l).There was no significant difference between onset and con-valescence of AMI. The influence of age on above results was eliminated by Logistic regression。Itwas showed that there was still a obvious difference on serum cystatin C concentration of AMIpatients from that of control. It was indicated that serum cystatin C concentration , in some de-gree,may be a reference for diagnosis of acute myocardial infarction.

关 键 词：心肌梗塞 巯基蛋白酶 CYSTATIN C 酶联免疫吸附 

分 类 号：R542.220.2[医药卫生—心血管疾病]