激光诱导杨树单性生殖的研究Ⅵ．子房愈伤组织的无性繁殖系及其分化培养  

STUDIES ON POPLAR PARTHENOGENESIS INDUCED BY LASER Ⅵ.Culture of the clone rfom the callus of ovary and its differentiation culture

作　　者：陈震古[1] 许成林[1] 刘小刚[1] 余皖苏[1] 

机构地区：[1]安徽农业大学生物技术研究中心激光研究室

出　　处：《激光生物学》1995年第4期730-732,726,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金

摘　　要：试验已获得用Ｈｅ－Ｎｅ激光诱导美洲黑杨“Ｉ６９”杨子房愈伤组织的无性繁殖系“ｃｌｏｎｅ１４”，经细胞学分析，证明它是起源于雌配子体的母系单倍体。经过两年来的继代培养，其愈伤组织的品质已越来越趋于纯化，用它的次生愈伤组织，在适合的分化培养基上，芽分化率由原来的２０％提高到５５％以上，而且切取分化根，进行芽分化培养，也能长芽。The asexual reproductive lines″clone 14″ of the ovary callus have been obtained ininducing populus deItoides Bartr CV. ″Lax″(I69)by He-Ne laser. The cytology analysisshowed that the″ clone 14″ was the maternal haploid which came from the female gameto-phyte. After two years’culture,the quality of the callus have become more and more pure.When its secondary callus vvere cultured on the proper differentiation medium,their bud dif-ferentiation rates have been raised to more than 55%from the original 20%。 Even more,itcan also grow bud from the differentiated roots which have been cut into differentiate buds.

关 键 词：激光 杨树 子房 组织培养 单性生殖 无性繁殖系 

分 类 号：S792.110.4[农业科学—林木遗传育种] S123[农业科学—林学]