丁二烯中毒大鼠脑细胞膜ATP酶活性变化的研究  

STUDY ON ATPase ACTIVITY OF CEREBRAL CELL MEMBRANE IN RATS EXPOSED TO 1,3-BUTADIENE

作　　者：马来记[1] 高树君[1] 厉保秋[1] 闫风琴 孙克任[1] 

机构地区：[1]山东医科大学毒理研究室

出　　处：《济宁医学院学报》1995年第2期7-9,共3页Journal of Jining Medical University

摘　　要：选择健康Ｗｉｓｔａｒ大鼠４０只，随机分为４组，每组１０只。静式吸入染毒，剂量分别为０、０．５、２．０和５．０ｍｇ／Ｌ丁二烯，每天４小时，每周６天，共１６周。断头处死大鼠，将头迅速投入液氮内速冻，并取出脑组织，Ｍａｔｓｕｍｕｒａ法制各膜蛋白，Ｂｒａｄｆｏｒｄ法进行蛋白定量，孔雀石绿法测定无机磷（Ｐｉ）．根据Ｐｉ和蛋白质的量，计算出ＡＴＰａｓｅ活性（ｎｍｏｌＰｉ／ｍｇ蛋白１０分钟）。结果表明脑组织神经细胞膜Ｎａ＋／Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋／Ｍｇ２＋－ＡＴＰａｓｅ活性增高，Ｎｅｕｒｏｆｉｌａｍｅｎｔｍｙｏｓｉｎ－ｔｙｐｅＡＴＰａｓｅ活性降低。提示脑细胞代谢活跃，神经轴突轴浆转运障碍，突触传递受阻。Wistar rats(male and female)were randomly separated into four groups(10 rats each group),which were exposed respectively to air containing 0、0.5、2.0、and 5.0mg/L 1,3-butadiene in the closed system,4 hours per dey,6 days per week,for 16 weeks. The rats were killed by cutting heads off,throwing them into liquid nitrogen immediately,and extracting brains.The membrane was prepared by a modified procedure as desecibed by Matsumura.Protein determination of memerane was by the method of Bradford.Pi was determined by the method of Kwol-Ming Chan,ATPase activity(nmol Pi/mg prot 10min)ws calculated on the basis of quantities of protein and Pi.The results of this study showed that Na+/K+-ATPase and Ca2+/Mg2+-ATPase activity of brain cell membrane were raised ;neurofilament myosin-type ATPase activity was reduced.The results indicated that the metabolism of brain cells was active;the axoplasmic transport of axon was damaged;synaptic transmission function was abnormal.

关 键 词：丁二烯 细胞膜 ATP酶 中毒 大鼠 

分 类 号：R595.02[医药卫生—内科学]