我国旋毛虫分离株同工酶分析  

ISOENZYME ANALYSIS OF TRICHINELLA SPIRALIS ISOLATES IN CHINA

作　　者：张月清[1] 石宏[1] 吴赵永[1] 王虹[1] 许炽[1] 

机构地区：[1]北京热带医学研究所

出　　处：《寄生虫与医学昆虫学报》1995年第2期85-90,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：北京市自然科学基金

摘　　要：本文对来自我国长春（自犬分离，简称Ｔ．ｓ６）、河南、天津、西安、河南和云南（均自猪分离，分别称Ｔ．ｓ１、Ｔ．ｓ２、Ｔ．ｓ３、Ｔ．ｓ４和Ｔ．ｓ５）旋毛虫６个分离株的８种同工酶进行了研究。Ｔ．ｓ６株与其他各分离株在５种同工酶（ＧＰＩ、Ｇ６ＰＤ、ＨＫ、６ＰＧＤＨ、ＡＫ）酶谱上存在着差异；在ＭＥ和ＰＧＭ的酶谱中完全相同；在ＭＤＨ酶谱中，Ｔ．ｓ６株与Ｔ．ｓ１、Ｔ．ｓ４两株酶谱相似，而与Ｔ．ｓ２、Ｔ．ｓ３、Ｔ．ｓ５不同。结果表明：犬分离株旋毛虫（Ｔ．ｓ６株）与源自猪的５个分离株旋毛虫的大多数同工酶酶谱存在明显差异。提示，我国旋毛虫虫株可能存在着两种不同的生物学类型。Six isolates of Trichinella spiralis isolated from Changchun (T.s6), Henan(T.s1 and T.s4),Tianjin (T.s2),Xian(T.s3)and Yunnan (T.s5)in China have been examined by the isoenzyme dectrophoresis for 8 isoenzymes(GPI, ME MDH, G6PD,HK,PGM, 6PGDH and AK).Changchun isolate was isolated from a naturally infected dog and the remaindere were from slaughtered swine naturally infected with T.spiralis. Changchun isolate was distinguishable from the remainders in isoenzyme patterns of GPI, G6PD, HK, 6PGDH and AK, while their isoenzyme patterns in ME and PGM were identical. The MDH pattern of Changchun isolate was similar to that of 2 Henan isolates,and it was distinguishable from that of Tianjin,Xian and Yunnan isolates. These results show that there are differences in isoenzyme patterns between Changchun isolate(from dog)and other five isolates (from swine).Thus it seems reasonable to assume that there are at least two different biological types of T.spiralis in China.

关 键 词：旋毛虫 分离株 同功酶 

分 类 号：R383.15[医药卫生—医学寄生虫学]