亚硫酸氢钠穿心莲内酯有关物质测定的方法学研究  

A HPLC assay for andrographolide sodium bisulfite and its relative substances

作　　者：金描真[1] 庞小雄[1] 易军[1] 郭波红[1] 汤佩莲[1] 张少瑜[1] 

机构地区：[1]广东药学院药科学院,广州510006

出　　处：《中国新药杂志》2005年第7期890-892,共3页Chinese Journal of New Drugs

摘　　要：目的:建立高效液相色谱法,测定亚硫酸氢钠穿心莲内酯的有关物质。方法:采用DiamonsilC18色谱柱(250mm×4·6mm,5μm),甲醇-乙腈-0·02mol·L-1磷酸二氢钾溶液(5∶24∶75)为流动相,220nm为检测波长。结果:亚硫酸氢钠穿心莲内酯有关物质与主峰分离度大于1·5,最小检出量为4·0ng。结论:本方法专属性好,简便快捷,可用于亚硫酸氢钠穿心莲内酯的质量控制。Objective：To establish a HPLC method for the determination of andrographolide sodium bisulfite and its relative substances. Methods： The samples were loaded and analyzed by HPLC, which consisted of a Diamonsil C18 column（250 mm × 4.6 mm,5 μm）, an eluant of methanol-acetonitfile-0.02mol·L^-1 KH2 P04 （5 ： 24 ： 75 ） and a detection at 220 nm. Results ： Andrographolide sodium bisulfite was separated from its relative substances well in a resolution ratio of 1 ： 5 or up. The detection limit was 4.0 ng. Conclusion： This specific and efficient HPLC method can be used for the quality control of andrographolide sodium bisulfite.

关 键 词：高效液相色谱法 亚硫酸氢钠穿心莲内酯 有关物质 质量控制 

分 类 号：R927.2[医药卫生—药学] R979.7