中药海蒿子多糖的分离纯化及结构研究  

THE STUDY OF THE ISOLATION, PURIFICATION AND STRUCTURE OF POLYSACCHARIDE FROM SARGASSUM PALLIDIUM

作　　者：赵宇[1] 张立新[2] 李志富[1] 任少红[1] 

机构地区：[1]泰山医学院化学与化学工程学院,泰安271000 [2]青岛大学化工学院,青岛266071

出　　处：《山东农业大学学报（自然科学版）》2005年第2期247-250,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：青岛市科委项目(No.02-1-kchhh-42-2P)

摘　　要：中药海蒿子干粉经氯仿脱脂、热水抽提、乙醇沉淀和DEAE-SephadexA-25柱层析分离,得到多糖SPⅠ、SPⅡ和SPⅢ,总糖含量分别为52.40%、51.60%、38.80%。用醋酸纤维薄膜电泳鉴定SPⅢ级分含有两种组分,SPⅠ和SPⅡ级分均为单一组分。紫外光谱对三种级分多糖溶液在220～400nm范围扫描,显示在260nm、290nm处SPⅠ、SPⅡ级分无核酸和蛋白吸收峰。红外吸收光谱表明三种级分多糖结构中均含β构型吡喃糖苷键。经酸降解、薄层层析和气相色谱-质谱分析,证明其单糖组成均为木糖、岩藻糖、半乳糖、果糖、甘露糖、葡萄糖,其中岩藻糖含量最高。The Polysaccharide SP Ⅰ ,SP Ⅱ and SPⅢ components were isolated from the sargassum Pallidium powder by Chloroform defatting, boiling water - extracting, ethanol - precipitation and DEAE - Sephadex A - 25 column chromatography. The content of total sugar were 52.40%, 51.60% and 38.80%. The SPⅢ showed two compoents in cellulose acetate membrane electrophoresis, while the SP Ⅰ and SP Ⅱcomponents showed single component. There were no nucleic acid and protein absorbance at 260nm and 290nm on the ultraviolet spectrum of SP Ⅰ and SP Ⅱ. The data of IR revealed that SP Ⅰ , SP Ⅱ and SP Ⅲ were chiefly linked by β- glycosidic bonds. The chemical composition analyzed by TLC and GC - MS showed that they were composed of xylose, fucoidan, galactose, fructose, mannose and the glucoses; and the fucoidan was primary component.

关 键 词：中草药 海蒿子 多糖 分离 纯化 结构坚定 β构型吡喃糖苷键 

分 类 号：Q949.28[生物学—植物学] R284.1[医药卫生—中药学]