自体骨髓单个核细胞移植联合益气活血中药治疗下肢动脉缺血性疾病23例  

Treatment of lower limb ischemia with combination of traditional Chinese medicine and transplantation of autologous bone marrow mononuclear cells: A report of 23 cases

作　　者：杨博华[1] 秦建辉[1] 朱龄群[1] 陈蕾[1] 路红[1] 刘凤桐[1] 李友山[1] 

机构地区：[1]北京中医药大学附属东直门医院血管外科,北京100700

出　　处：《中西医结合学报》2005年第1期28-30,共3页Journal of Chinese Integrative Medicine

基　　金：国家中医药管理局科研基金资助项目 (No .0 4 0 5LP0 2 )

摘　　要：目的 :探讨自体骨髓单个核细胞移植与中药合用对下肢缺血的治疗作用。方法 :治疗对象为 2 3例严重下肢缺血患者。术前用集落细胞刺激因子使患者骨髓处于增生活跃状态 ,抽取骨髓液后分离单个核细胞 ,细胞总量达到 1× 10 9以上。移植采用缺血肢体多点注射。移植后 1d起服益气活血中药。观察患者手术前后肢体疼痛、冷感评分、踝 /肱指数 (ankle/brachialindex ,ABI)以及溃疡创面情况。结果 :治疗后患肢疼痛及冷感评分逐渐降低 ,治疗 1个月后与治疗前比较有显著性差异 (P <0 .0 5 ) ;ABI在治疗后逐渐升高 ,治疗后 1个月平均升高了 0 .15。而足部溃疡面积改善不明显。结论 :自体骨髓单个核细胞移植结合中药治疗可以迅速改善下肢缺血患者的疼痛及冷感 ,能有效改善局部的血液循环。Objective： To evaluate the therapeutic effect of transplantation of autologous bone marrow mononuclear cells combined with traditional Chinese medicine for the treatment of limb ischemia. Methods：Twenty-three patients with limb ischemia were treated. G-CSF was used to stimulate the bone marrow. The mononuclear cells were separated from the aspirated bone marrow fluid in the stem cell studio. The cell amount was above 1 x 109. The transplantation was performed by the way of intra-muscular multi-injection.Traditional Chinese medicine for replenishing qi to activate blood was prescribed from the first day after operation. The pain, poikilothermia, ulcer or necrosis and ankle/brachial index （ABI） of the ischemic limb were evaluated before and after the treatment. Results： The pain score and poikilothermia score decreased one month after the transplantation, with distinct differences as compared with the scores before the treatment （ P 〈0.05）. The ABI increased gradually after the treatment, and one month after the treatment, it was 0.15 higher than that before the treatment. Conclusion： Transplantation of autologous bone marrow mononuclear cells combined with traditional Chinese medicine can decrease the symptoms and signs of severe lower limb ischemia effectively, and improve the circulation of the ischemic area.

关 键 词：骨髓移植 单个核细胞 下肢缺血 中医药疗法 

分 类 号：R543.6[医药卫生—心血管疾病]