牛珀至宝微丸对内毒素休克大鼠脑神经型一氧化氮合酶表达的影响  

Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats

作　　者：周健洪[1] 陈东风[1] 杜少辉[2] 黎晖[1] 李伊为[1] 邓汝东[1] 张赛霞[1] 

机构地区：[1]广州中医药大学解剖学教研室,广东广州510405 [2]深圳市中医院内科,广东深圳518000

出　　处：《中西医结合学报》2005年第2期115-118,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.39970914);广东省中医药管理局基金资助项目(No.98447);深圳市科技局基金资助项目No.200004087)

摘　　要：目的:探讨牛珀至宝微丸对内毒素休克大鼠脑神经型一氧化氮合酶(neuronalnitricoxide synthase,nNOS)表达的影响。方法:将SD大鼠随机分成正常对照组、模型组、牛珀至宝微丸组。模型组静 脉注射内毒素脂多糖(lipopolysaccharide,LPS)1.5mg/kg、腹腔注射D 氨基半乳糖(D galactosamine, D GalN)100mg/kg造成内毒素休克模型,牛珀至宝微丸组用药7d后再作以上处理。用免疫组织化学方法 检测各组nNOS在不同脑区的表达。结果:nNOS阳性细胞广泛分布于大脑皮质Ⅱ、Ⅲ、Ⅳ层,海马分子层, 齿状回多形层,脑干网状结构,小脑分子层、颗粒层和普尔基涅细胞层。在大脑皮质、海马、脑干及小脑各部 位,牛珀至宝微丸组的nNOS阳性细胞数略高于正常对照组,但明显低于模型组(P<0.05)。结论:牛珀至 宝微丸有部分下调内毒素休克所致广泛脑区nNOS过度表达的作用。Objective： To investigate the effect of Niupo Zhibao Pellet （NPZBP） on the expression of neuronal nitric oxide synthase （nNOS） in the brain of endotoxin-induced shock rats. Methods： SD rats were randomly divided into normal control group, endotoxin-induced shock model group and NPZBP-treated group. Lipopolysaccharide （LPS）（ 1.5 mg/kg i. v. ） and D -galactosamine （ D -GAIN） （ 100 mg/kg i. p. ） were administered to the rats in endotoxin-induced shock model group, as well as to the rats in NPZBP-treated group after seven-day treatment, to induce the shock. The expression of nNOS in the brain of the rats in each of the 3 groups was measured by immunohistochemicalmethods. Results： In the 3 groups, nNOS immuno-positive cells distributed widely in layer Ⅱ, Ⅲ, Ⅳ of the cerebral cortex, the molecular layer of hippocampus, the polymorphic layer of the dentate gyrus, the reticular formation of brain stem, and the molecular, granular and Purkinje cell layer of the cerebellar cortex. The number of immuno-positive cells in the NPZBP-treated group was slightly higher than that of the normal control group, and signifficantly lower than that of the model group （P〈0.05） in many regions of the brain, including cerebral cortex, hippocampus, brain stem and cerebellarcortex. Conclusion： NPZBP can inhibit the over-expression of nNOS in wide area of the brain in endotoxin-induced shock rats.

关 键 词：牛珀至宝微丸 内毒素休克 神经型一氧化氮合酶 免疫组织化学 动物 实验 

分 类 号：R285[医药卫生—中药学]