三七提取物对MNNG转化后GES-1细胞的促凋亡作用  

Apoptosis-promoting effect of Panax notoginseng extracts on MNNG-transformed GES-1 cells

作　　者：李军祥[1] 王志斌[1] 朱陵群[2] 牛福玲[2] 崔巍[2] 

机构地区：[1]北京中医药大学东方医院消化内科,北京100078 [2]北京中医药大学东直门医院中医内科学重点实验室,北京100700

出　　处：《中西医结合学报》2005年第2期123-127,共5页Journal of Chinese Integrative Medicine

基　　金：教育部科学技术研究重点资助项目(No.2024)

摘　　要：目的:应用流式细胞测定法研究灌服三七提取物后的犬血清对胃癌前细胞的促凋亡作用。方法:采 用被N 甲基 N’ 硝基 N 亚硝基胍(N methyl N’ nitro N nitroso guanidine,MNNG)进一步转化后的永 生化人胃黏膜上皮细胞系GES 1细胞(以下简称MC细胞)作为胃癌前病变细胞的体外研究模型;以三七水 提物给彼格犬一次性灌胃,取给药前的血清、给药后2h和6h的血清作为实验药物血清。以流式细胞测定 法分别检测2h和6h两个采血时间点上不同浓度的药物血清对MC细胞作用72h后的促凋亡作用。结 果:这两个时间点的含药血清能明显促进MC细胞的凋亡,与同样条件下用空白血清培养的MC细胞相比 其凋亡率显著增加(P<0.05)。在两个时间点的药物血清作用下的MC细胞,其G0/G1期细胞比例明显下 降(P<0.05),而G2/M期细胞比例显著增加(P<0.05),但S期细胞比例并没有一致性的变化。结论:两 个时间点的药物血清对MC细胞都有显著的促凋亡作用,且能显著降低G0/G1期的MC细胞比例及显著增 加G2/M期的MC细胞比例,这可能是其抑制MC细胞增殖及促凋亡的原因之一。Objective： To study the apoptosis-promoting effect of the serum from Panax notoginseng extracts-fed dog on precancerous gastric cells by means of flow cytometry. Methods： In the experiment, we adopted eternalized human gastric mucosa epithelium GES-1 cells transformed by N-methyl-N＇-nitro-N-nitroso-guanidine （MNNG） （MC cells） as the model of precancerous lesions for study in vitro. We took the serum of a dog before and at two different points of time （2 and 6 hours） after feeding the dog with Panax notoginseng extracts for experiment. The MC cells were cultured in mediums with different concentrations of the medicated serum at 2- or 6-hour point of time for 72 hours. By means of flow cytometry, we examined the apoptosis-promoting effects of the serums on the MC cells. Results： The medicated serums at these 2 points of time had significant effects in promoting MC cell apoptosis. The proportions of apoptotic cells in culture mediums with medicated serums had a significant increase as compared with those in culture mediums with non-medicated serums （serum obtained before administration of extracts to the dog） under the same conditions （P〈0.05）. The number of MC cells in Go/G1 phase was decreased （P〈0.05） and that in G2/M phase increased （P 〈 0. 05）, while no consistent changes were observed in S phase. Conclusion： Themedicatedserums obtained at the two different points of time have significant apoptosis-promoting effects on MC cells. They decrease the number of MC cells in Go/G1 phase and increase the number of MC cells in G2/M phase. This is probably responsible for the effects of Panax notoginseng extracts in inhibiting the proliferation of MC cells and promoting its apoptosis.

关 键 词：三七 胃癌 细胞培养 凋亡 动物 实验 

分 类 号：R285.6[医药卫生—中药学]