膜芯片检测人B组、C组轮状病毒的初步研究  

Study of method of membrane gene chip for detection of human B and C rotavirus

作　　者：黄海燕[1] 韩金祥[1] 王健伟[2] 朱波[1] 彭丽[3] 

机构地区：[1]山东省医药生物技术研究中心,山东济南250062 [2]山东省病毒学研究所 [3]山东省肿瘤防治研究院

出　　处：《山东医药》2005年第20期1-3,共3页Shandong Medical Journal

基　　金：国家"十五"计划资助项目(2001BA804A22)

摘　　要：目的建立检测人B组、C组轮状病毒(RV)的膜芯片。方法采用化学合成法合成B组、C组RV高度保守的核酸扩增区域,以地高辛标记上游引物,经半巢式PCR扩增B组、C组RV的保守检测区域,采用本实验室自行研制的膜芯片进行杂交检测。结果B组、C组RV的扩增产物同膜芯片上特异探针呈高度特异杂交。结论自制的膜芯片检测技术能够实现B组、C组RV的检测,并具有特异性好、高通量、平行性和简便快速的特点。Objective,To develop a method of membrane gene chip for detection of human B and C rotavirus.Methods：To synthesize the highly conservative nucleotide sequences of group B and group C rotavirus and mark the up-stream prime with DIG, the conservative region for detection were amplified by semi-nested PCR. Then the PCR products were detected by hybridizing with membrane chips respectivly. Results：The products of B,C rotavirus can hybridize with the probes immobilized on the membrane chip with high specificity. Conclusion：This type of membrane chips can be used to detect human B and C rotavirus,which is distinctive,robust,parallel ,quick and simple.

关 键 词：轮状病毒 检测 芯片 杂交 

分 类 号：R446[医药卫生—诊断学]