河北省鼠疫菌株生化特性的研究  

The Research of Yersinia pestis Biochemical Characters in Hebei

作　　者：刘合智[1] 张月芝[1] 史献明[1] 杜国义[1] 白小英[1] 陈志新[1] 刘秀莲[1] 董国润[1] 崇岩岷[1] 

机构地区：[1]河北省鼠疫防治所检验科,张家口075000

出　　处：《中国媒介生物学及控制杂志》2005年第4期308-310,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的通过测定2002～2003年河北省鼠疫自然疫源地分离的27株鼠疫菌,得到其生化特性的本底资料。方法采用液体汤管法和固体试管法(斜面或高层)实验测定27株鼠疫菌对糖醇的酵解能力和生化反应。结果(1)27株鼠疫菌对甘油、L阿胶糖等多种糖酵解,产酸不产气;不发酵麦芽糖、鼠李糖,72h内对密二糖不酵解,5d以后有8株菌的部分糖管呈迟缓发酵。(2)生化反应:27株鼠疫菌在还原KNO3、硝化反应、VP试验、尿素试验、靛基质试验呈阴性,不液化明胶,在无蛋白胨酸性琼脂培基上不生长,不能利用枸橼酸盐;甲基红反应阳性,均能轻微产生H2S。结论被试的27株鼠疫菌按中国鼠疫菌生态型分型标准属于B群鄂尔多斯高原型。主要生化特征稳定,与疫源地过去分离的鼠疫菌株生化特性基本一致。Objective To research the biochemical characters of 27 Yersinia pestis, so as to get their basic data of biochemical information. Methods To determine the capability 27 plague strains to ferment carbohydrate-alcohols and biochemical reaction of with test-tube method of liquid nutrient medium and solid culture medium （slant or high-layer）. Results （1）The tested Y. pestis fermented Glycerol, L-Arabinose,etc. which was acidgenic and aerogenic and didn＇t ferment Maltose,Rhamnose. Fermenting Melibiose was negative in 72 h and part ferments-tube of 8 strains appeared positive after 5 d. （2）Biochemical reaction： The results of 27 strains showed negative reaction in the reducing KNO3, nitrate reaction, V-P experiment, urea decomposition, indole formation, liquefies gelatin, utilizes citrate and growth test on non-peptone acid agar medium. Methyl red experiment was positive and they were able to slightly produce H2S. Conclusion According to Chinese Y. pestis ecotype typing standard, the strains belong to B group, Erduosi Plateau, and their principal biochemical characters correspond with Y. pestis isolated from the focus in the past.

关 键 词：河北 鼠疫菌 生化特性 汤管法 

分 类 号：R516.8[医药卫生—内科学]