昆虫细胞培养研究进展  

Progress in Insect Cell Culture

作　　者：李守信[1] 李长友[2] 郑桂玲[2] 周洪旭[2] 吴文君[1] 李国勋[2] 

机构地区：[1]西北农林科技大学农药研究所,陕西杨凌712100 [2]山东莱阳农学院害虫生防实验室,山东青岛266109

出　　处：《西北农业学报》2005年第3期41-48,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(30370963)

摘　　要：随着生命科学的迅速发展,细胞工程愈来愈受到人们的重视。以昆虫细胞为对象的细胞培养技术在现代实验生物学上具有重要的价值,已经广泛地应用于医学、农业及生物学的各个领域。本文综述了有关昆虫细胞培养的研究进展,包括昆虫细胞培养基研究开发,昆虫细胞系的建立和组织培养,利用生物反应器大规模培养昆虫细胞,昆虫细胞-杆状病毒表达系统(B acu lov irus express ion vector system,BEV S),构建基因工程细胞系及其稳定性表达,以及昆虫细胞培养的应用前景和研究展望。With life sciences developing rapidly ,cell engineering need to pay particular attention, insect cell culture have become important tools in biological research area, it has been widely used for the studies of physic ,agriculture and biology. The progress in insect cell culture was overviewed in this paper including the research of insect cell culture medium, establishment of insect cell lines,bioreactor, insect cell-baculovirus expression system, and construction of transfected gene cell lines,the application prospect and future development of insect cell culture were also discussed.

关 键 词：昆虫细胞系 昆虫细胞培养 基因表达 组织培养 昆虫细胞-杆状病毒表达系统 

分 类 号：Q813.11[生物学—生物工程]