转草酸氧化酶基因的甘蓝型油菜(Brassica napus L.)的苗期人工春化条件探索  

Study on Artificial Vernalization Condition of Rapeseed(Brassica napus L.) with Oxalate Oxidase Gene

作　　者：孙燕飞[1] 康振生[1] 张岗[1] 韩青梅[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《西北农业学报》2005年第3期67-69,72,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省农业分子生物学重点实验室基金资助项目;陕西省自然科学基金项目(2004C101)

摘　　要：对4种转草酸氧化酶基因甘蓝型油菜(Brassica napus L.)苗期人工春化条件进行研究的结果表明,春性和冬性半冬性转基因油菜移栽后,4～6叶期经适当低温处理后均可正常开花结实;转基因苗不移栽直接在试管中经适当低温处理也可以通过春化阶段;春性转基因油菜的春化处理时间长短与开花时间早晚有关,春化处理时间最长的,开花也最早,与开花株率关系不大;冬性半冬性转基因油菜的开花株率与春化处理时间长短呈正相关;4～6叶期转基因半冬性油菜通过春化阶段需时35～40 d以上,转基因冬性油菜通过春化阶段需时50 d,且适当延长春化时间能促进花期提前.Artificial vernalization technique of four oriental cultivars of transgenic rapeseeds （Brassica napus L. ） in seedling stage was studied. The results showed that： spring and winter rapeseed（Brassica naus L. ）, in their four or six-leaf stage, can flower by proper low temperature treatment days, as well as aseptic seedlings. The flowering rate of the spring rapeseed was less influenced by the treatment days, while that of the winter and semi-winter rapeseed was influenced by the treatment days; Semi-winter rapeseeds （]3rassica napus L. ） at four or six -leaf stage needed 35 - 40 days treatments to pass vernalization, and the effect was improved by prolonging treatment days, while winter rapeseeds needed 50 d.

关 键 词：甘蓝型油菜 转基因 试管苗 人工春化 

分 类 号：S565.4[农业科学—作物学]