三叶木通总DNA提取方法的比较  

Comparative Study of Methods in Isolation Total DNA from Akebia trifoliata Koidz.

作　　者：张铮[1] 王强[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《西北农业学报》2005年第3期141-144,共4页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：以三叶木通(Akebia trif olia ta K o idz.)的新鲜叶片和硅胶干燥叶片为材料,分别采用CTAB法、高盐低pH法和苯酚法3种不同方法提取其植物总DNA,用琼脂糖凝胶电泳和紫外分光光度法检测获得的DNA的质量,以期从中找出提取三叶木通植物总DNA的最适方法。结果表明对于三叶木通的新鲜叶片和硅胶干燥叶片,无论从产率上还是质量上,CTAB法都明显优于其他两种方法。The total DNA was isolated from the fresh leaf and the leaf dried fast by silica gel of Akebia Trifoliata Koidz. with methods of CTAB, high salt and phenol respectively. The quality of the DNA was examined with agarose gel electrophocesis and sepectrophotometry. Then the results were compared so as to find an optimum method in extracting the total DNA from A. trifoliata. The results suggested that the method of CTAB is obviously better than the other two methods.

关 键 词：三叶木通 植物总DNA 提取方法 新鲜叶片 硅胶干燥叶片 产率 质量 

分 类 号：S567.19[农业科学—中草药栽培]