参七汤影响缺血心肌内皮素基因表达的实验研究  

The Effects of Shenqi Decoction(参芪汤) on the Gene Expression of Endothelins in Rats with Myocardium Ischemia

作　　者：赵华云[1] 蔡军[2] 秦鉴[3] 黄洁玲[1] 郎江明[1] 金明华[3] 

机构地区：[1]广东省佛山市中医院,佛山528000 [2]广东省始兴县人民医院,始兴512500 [3]中山大学附属第一医院,广州510080

出　　处：《中国中医急症》2005年第8期764-765,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：广东省中医药局科研基金资助课题(No.100013)

摘　　要：目的研究参七汤对心肌缺血大鼠心肌内皮素影响的分子机制。方法将SD大鼠随机分为正常对照组、缺血组和参七汤组,利用垂体后叶素造模,分别测定各组心肌内皮素浓度及内皮素-1基因的表达。结果参七汤组心肌内皮素浓度明显低于缺血组;免疫组化显示缺血组心肌内皮素-1染色灰度值明显低于对照组和参七汤组。结论参七汤可显著降低缺血心肌内皮素浓度,可能是参七汤所代表的益气补元活血法能有效抑制缺血心肌内皮素-1基因的表达及蛋白合成。Objective:To study molecule mechanism of Shenqi Decoction (SD,参芪汤) on endo thelins(NO),in rats with myocardium ischemia.Methods:SD rats were divided into t he control group,the ischemica group and the SD group.Took advantage of pituitri n to make rat ischemic model,and assayed NO concentration and NO-1 gene express ion in myocardium every group.Results:NO concentration of the SD group was signi ficantly lower than that of the ischemia group,and immunohistochemical method di splayed thet NO-1 staining gray value of the ischemia group was evidently lower than that of the SD group.Conclusions:The reason that the SD prominently reduce d NO concentration of ischemic myocardium might be that it could effectively res train NO gene expression and protein synthesis in myocardium,which represents th e treatment method of Supplement Qi,Reinforcing Primordium and Activating Blood Circulation.

关 键 词：参七汤 缺血心肌 内皮素 基因表达 中医药疗法 

分 类 号：R259.422[医药卫生—中西医结合]