水溶性荧光聚合物用于测定聚赖氨酸  

Determination of Poly-L-lysine with a Water-soluble Fluorescence Polymer

作　　者：陈彦国[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院

出　　处：《高等学校化学学报》2005年第8期1428-1431,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20275028);国家"八六三"计划项目(批准号:2002AA2Z2004)资助.

摘　　要：合成了一种水溶性荧光聚合物聚[5-甲氧基-2-(3-磺酰化丙氧基)-1,4-苯撑乙烯](MPS-PPV),对该聚合物进行了元素分析、红外光谱及透射电子显微镜表征.实验结果表明,聚赖氨酸(PLL)对该聚合物荧光具有明显的猝灭作用,据此建立了一种快速灵敏测定聚赖氨酸的新方法,检出限为5.0×10-10mol/L.探讨了聚赖氨酸猝灭MPS-PPV荧光的机理.A water-soluble fluorescence polymer poly [ 5-methoxy-2- （3-sulfopropoxy）-1, 4-phenylenevinylene] （MPS-PPV） was synthesized and characterized by elemental analysis, infrared absorption spectrun and the transmission electron microscope micrograph. The fluorescence of MPS-PPV could be quenched by poly-L-1ysine（PLL）, a new food preservative. So a rapid and sensitive method for the determination of PLL was set up, the linear range and detection limit were 3.6 × 10^-9 - 2.5 × 10^-8 mol/L and 5.0 × 10^-10 mol/L respectively. The fluorescence quenching mechanism of PLL to MPS-PPV was also discussed, which was caused by the strong electrostatic interaction between cationic PLL and anion conjugated polymer MPS-PPV.

关 键 词：荧光 聚合物 聚[5-甲氧基-2-(3-磺酰化丙氧基)-1 4-苯撑乙烯] 聚赖氨酸 

分 类 号：O625[理学—有机化学]