聚(L-丙氨酸)-聚羟乙基谷氨酰胺双嵌段两亲性共聚多肽的合成及其在水溶液中的自组装  

Synthesis of Amphiphilic Diblock Copolymer Poly(L-alanine)-b-poly(hydroxyethyl glutamine) and Its Self-assembly in Water

作　　者：李丽颖[1] 孙平川[1] 要旸[1] 陈铁红[1] 李宝会[2] 金庆华[2] 丁大同[2] 

机构地区：[1]南开大学化学学院 [2]南开大学物理系,天津300071

出　　处：《高等学校化学学报》2005年第8期1548-1551,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20373029;20374031;20233030);南开大学-天津大学纳米共建项目资助.

摘　　要：利用光气法分别以L-谷氨酸和L-丙氨酸为原料,合成了γ-谷氨酸苄酯-NCA单体和L-丙氨酸-NCA单体,再以三乙胺为引发剂,合成了聚(L-丙氨酸)-聚(γ-谷氨酸苄酯)(PLA50-b-PBLG20)双嵌段共聚多肽,并用乙醇胺对其中的PBLG嵌段进行亲核取代,把疏水性的苄酯侧链变为亲水性的羟烷酰胺侧链,得到双亲性的聚(L-丙氨酸)-聚羟乙基谷氨酰胺(PLA-b-PHEGA)双嵌段共聚多肽.利用红外光谱和核磁共振谱对产物进行了表征,用TEM研究了双嵌段共聚多肽PLA50-b-PHEGA20在水溶液中的自组装.研究结果表明,双嵌段共聚多肽在水溶液中可自组装形成囊泡.Two kinds of amino acid N-carboxyanhydrides γ-benzyl-glutamate N-carboxyanhydride（GluBNCA）, and L-alanine N-carboxyanhydride （Ala-NCA） were synthesized according to phosgene method （Fuchs-Farthing method ）. Poly （L-alanine）-block-poly （γ-benzyl-glutamate） （PLA-b-PBLG） AB diblock copolypeptide was synthesized by ring opening polymerization of Ala-NCA and GluB-NCA, with triethylamine as the initiator. The poly （L-alanine）-b-poly （hydroxyethyl glutamine） （PLA-b-PHEGA） diblock copolypeptide was obtained by reacting the PLA-b-PBLG diblock copolypeptide with ethanolamine to improve the hydrophilicity of PBLG block. The structure of the product was characterized by means of FTIR and ^1H NMR. The aggregation morphologies of the PLA-b-PHEGA diblock copolypeptide in aqueous solution were observed by TEM. It was found that the diblock copolypeptide could be self-assembled to form vesicles in water.

关 键 词：聚谷氨酸苄酯 聚丙氨酸 嵌段共聚物 自组装 

分 类 号：O631[理学—高分子化学]