家蚕基因特异性CAPs标记获得及其分子系统学应用  

The Acquisition of Two Silkworm CAPs Markers and Their Use in Genetic Diversity and Phylogenetic Relationship

作　　者：黄健华[1] 苗雪霞[1] 李木旺[1] 张勇[1] 赵卫国[2] 黄勇平[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]中国农业科学院镇江蚕业研究所,镇江212018

出　　处：《遗传》2005年第4期584-588,共5页Hereditas（Beijing)

基　　金：上海市科学技术委员会基础研究重点项目基金(编号:03JC14077);中科院重要方向项目(编号:KSCX2SW318)~~

摘　　要：选取家蚕attacin和alphaamylase基因序列,设计特异性引物,在家蚕品系P50、C108和子一代(F1)中扩增。分别采用4种不同的限制性内切酶对扩增产物酶切,最后每个基因都获得了一个CAPs分子标记。依据所得的两个CAPs分子标记对12个品系的家蚕遗传多样性进行了初步研究,构建了其分子系统树。Cleaved amplified polymorphic sequence （CAPs） markers are based on PCR amplification of known genes,cDNA sequences or RAPD sequences. The PCR products are digested by restriction enzymes, generating the simple type of data as heterozygotes and homozygotes. Here we designed primers based on silkworm attacin and alpha-amylase genes,then digested the PCR products in silkworm strains P50, O108 and their progeny F, using 4 different restriction enzymes respectively. Furthermore,the genetic diversity and phylogenetic relationship of 12 silkworm strains were investigated using the obtained two CAPs markers.

关 键 词：家蚕 CAPS标记 多态 酶切 分子系统树 

分 类 号：S881.26[农业科学—特种经济动物饲养]