早熟禾品种间遗传多样性分析  

Genetic Polymorphism of Bluegrass Cultivars Detected by RAPDs

作　　者：宁婷婷[1] 张再君[1] 金诚赞 朱英国[1] 

机构地区：[1]武汉大学植物发育遗传学教育部重点实验室,武汉430072 [2]朝鲜国家科学院生物分院植物研究所

出　　处：《遗传》2005年第4期605-610,共6页Hereditas（Beijing)

摘　　要：草地早熟禾(PoapratensisL.)是一种重要的冷季型草坪草,抗逆性强,适应性广,株型低矮,绿色期长,并且在良好的管理条件下,特别适合用作运动场草坪。近几年来在我国的园林绿化和运动场建植中得到了广泛的应用,现在已经引进的品种多达上百种。文章选用28个随机引物对15个草地早熟禾品种和1个加拿大早熟禾品种进行RAPD分析,25个引物共扩增出218条带,多态性条带比率为89.91%。并且对每个品种的几项坪用特性进行了观察。聚类分析结果表明,草地早熟禾品种之间的遗传多样性较低,相似性集中在60.76%～98.52%。加拿大早熟禾的一个品种单成一支,与其他草地早熟禾的品种相似性较低。品种之间的聚类关系与表型特征呈现不完全相关性。Kentucky bluegrass （Poe pretensis L. ）is a hardy, persistent forage and turf grass adapted to a wide range of soils and climates. Its ever-increasing adoption in highly cared-for sports fields has attracted the attention of many seed companies. However in the past, the breeding of elite varieties was often hampered by the extreme complexity of the genome. The polymorphism is important for broading the genetic basis and may be exploited for application of heterosis. The genetic relationship of 16 bluegrass cultivars, including 15 accessions Kentucky bluegrass cultivars and 1 entries Canada bluegrass （ Poe compressa L. ） cultivar from different breeding companies were analyzed using 25 RAPD markers. 25 RAPD primers generated 218 bands, of which 196 bands （89.91% ）were polymorphism. It showed that the Canada Bluegrass was separated from other Kentucky Bluegrass and genetic polymorphism in the Kentucky Bluegrass cultivars was low, the genetic similarity among the cultivars fell between 66 % -98 %. Dendrogram obtained using these molecular markers were partly in agreement with their separated morphologic character. Cultivars from the same company were not clustered in one group.

关 键 词：早熟禾 品种 遗传多样性 草坪草 聚类关系 表型特征 

分 类 号：S688.4[农业科学—观赏园艺]