晶体蛋白基因的转录调控与其超螺旋构形的关系  

INVOLVEMENT OF SUPERHELICAL STRUCTURE IN CONTROLLING THE REGULATORY TRANSCRIPTION OF CRYSTALLIN GENE

作　　者：刘祥麟[1] 陈亨[2] 

机构地区：[1]浙江医科大学肿瘤基因室 [2]纽约大学生物化学系

出　　处：《浙江医科大学学报》1989年第1期1-6,共6页

摘　　要：本文在一个克隆化的基因组系统中,研究了DNA超螺旋结构在α-晶体蛋白基因表达中的调控作用;测定了调节基因区及其对转录过程的影响.结果显示α-晶体蛋白基因可被RF-36蛋白因子特异性激活.足迹法分析表明其调控结合区为核苷酸“-AGGGCTGGAA-”,能与特定的DNA配位体结合,例如抗Z-DNA单克隆抗体和Chiral金属复合物,此种结合表明α-晶体蛋白基因富含“GC”盒,并具有类似“Z”型的DNA构型.所获结果还提示,DNA高度有序的结构在α-晶体蛋白基因的表达调控过程中起重要作用.The role of DNA superhelical structure in the regulation of α-crystallin gene expression in a cloned genomic system has been studied. In the experiment reported in this paper, we determined the regulatory gene domain and its effect on transcription. The transcription reactions were carried out in wheat germ pol Ⅱ and the HeLa cell extract system. The incorporation of ^(32)P-UMP into RNA transcripts was measured. The results has indicated that the α-crystallin A_2 gene was specifically activated by RF- 36 protein factor. Footprint analysis has revealed the regulatory binding domain to be a nucleotide sequence of '-AGGGCTGGAA-'.Its interaction with specific DNA binding ligands such as anti-Z-DNA monoclonal antibody and the chiral metal complex indicates that the 'GC' rich box possesses a conformation similar to 'Z' form DNA. It has also been demonstrated that the high order structure of DNA plays a major role in the regulation of the expression of the α-crystallin gene.

关 键 词：晶体蛋白 螺旋结构 转录调控 

分 类 号：Q343.15[生物学—遗传学]