人类ERMAP基因在诱导K562细胞向红系和巨噬系细胞分化过程中表达的研究  

Study on the Expression of Human ERMAP Gene in Erythropoietic and Macrophage Differentiation of K562 Cells

作　　者：何映谊[1] 张晓红[1] 叶铁真[1] 吴梓梁[1] 

机构地区：[1]广州医学院第一附属医院儿科,广州510120

出　　处：《中国实验血液学杂志》2005年第4期553-556,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30070797

摘　　要：为了探讨人类ERMAP基因在红细胞分化发育过程中的作用,以Ara-C诱导K562细胞向红系方向分化,以十二氟波酯钠盐(TPA)诱导K562细胞向巨噬系方向分化,用荧光定量PCR检测上述过程中人类ERMAP基因表达量的变化。结果发现:终浓度为2.5×10-6mmol/L/L及1.0×10-6mmol/L/L的Ara-C可诱导K562细胞向红系方向分化,在此过程中人类ERMAP基因的表达量逐渐增加;终浓度为2.0×10-6mmol/L/L及1.0×10-6mmol/L/L的TPA可诱导K562细胞向巨噬系方向分化,在此过程中人类ERMAP基因的表达量无变化。结论:人类ER-MAP基因与红系分化增殖密切相关。In order to investigate the potential of human ERMAP gene in erythroid cell differentiation, K562 cells were induced to erythroid lineage by Ara-C and to macrophage lineage by TPA, human ERMAP mRNA was detected by fluorescent quantitative PCR. The results showed that human ERMAP mRNA increased while K562 cells were induced to erythroid lineage after treatment with Ara-C at 2.5 ×10^-6 mmol/L/L and 1.0× 10^-6 mmol/L/L. Human ERMAP mRNA not changed while K562 cells were induced to macrophage lineage after treatment with TPA at 2.0 × 10^ -6 mmol/L/L and 1.0 × 10^-6 mmol/L/L. It is concluded that human ERMAP gene plays an important role in differentiation and proliferation of erythroid cells.

关 键 词：ERMAP基因 K562细胞 红系细胞 巨噬细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R733.7[医药卫生—基础医学]