免疫磁珠选择性清除人异基因反应性T细胞的实验研究  

Selective Depletion of Donor Alloreactive T Cells by Using Immunomagnetic Cell Sorting

作　　者：吕慧[1] 柴忆欢[1] 徐杰[1] 

机构地区：[1]苏州大学附属儿童医院血液科,苏州215003

出　　处：《中国实验血液学杂志》2005年第4期637-640,共4页Journal of Experimental Hematology

基　　金：江苏省卫生厅重点课题基金资助项目;编号:H200105

摘　　要：本研究用磁性细胞分离系统清除CD25+和CD69+人同种异体反应性T细胞,为减轻异基因骨髓移植后的移植物抗宿主病探索新的手段。对健康供者外周血单个核细胞与白血病缓解病人的骨髓单个核细胞进行单向混合淋巴细胞培养。3天后,通过磁性细胞分离系统清除CD25+和CD69+的同种异体反应性T细胞;通过3H-TdR掺入实验,观察处理后的供者外周血单个核细胞对同一病人的骨髓单个核细胞及无关者外周血单个核细胞的反应性。结果表明:与未清除组相比,清除后供者细胞对同一病人抗原的反应性降低50%,但保留了大部分对无关者抗原的反应性。结论:在体外反应中清除同种异体反应性T细胞可降低对刺激抗原反应,同时保留大部分对无关者抗原的反应。This study was aimed to explore a new method of alleviating graft-versus-host disease （GVHD） after allogeneic bone marrow transplantation through selective elimination of human alloreactive T cells expressing either CD25^＋ or CD69^＋ by immunomagnetic cell sorting （MACS）. Healthy donor peripheral blood mononuclear cells were cocultivated with bone marrow mononuclear cells from HLA-nonidentical leukemia recipient with remission in one-way mixed lymphocyte culture （OWMLC）. After 3 days, both CD25 ^＋ and CD69^＋ lymphocytes were removed by MACS. The depleted donor fraction and untreated donor cells were then rechallenged in a secondary mixed lymphocyte culture （MLC）with the original stimulator cells or a third party to assess relative alloreactivity. The results showed that 50% inhibition of the secondary MLC was observed in the depleted donor fraction. Alloreactivity against unrelated third-party cells was largely preserved. It is concluded that this method reduces alloreactivity while retaining reactivity against a third party target in vitro.

关 键 词：骨髓移植 移植物抗宿主病 选择性T细胞清除 免疫磁性细胞分离系统 

分 类 号：R457.7[医药卫生—治疗学]