建立人/猪造血嵌合体模型的初步探讨  

A Pilot Study on Establishment of Human/Pig Hematopoietic Chimera Model in Fetal and Neonatal Pigs

作　　者：檀英霞[1] 王捷熙[1] 李明 章扬培[1] 

机构地区：[1]军事医学科学院野战输血研究所血液生化与分子生物学研究室,北京100850 [2]北京市SPF猪育种中心,北京100095

出　　处：《中国实验血液学杂志》2005年第4期673-676,共4页Journal of Experimental Hematology

基　　金：国家"973"项目;编号(2002CB713804)

摘　　要：本研究目的是利用免疫系统尚未成熟的胎猪和新生猪作为人脐血造血干细胞(HSC)的移植受者,建立人/猪造血嵌合体模型,初步探讨在猪体内扩增HSC的可行性。在无菌隔离器中,通过肌肉或脐静脉(剖子宫)给胎猪移植人脐血细胞,或在断脐前通过脐静脉给新生猪移植人脐血细胞,应用流式细胞术检测移植组猪外周血中人CD45+细胞。结果表明:移植后,受试猪生长发育与对照猪一致;移植后60天时,用流式细胞术在猪的外周血中检测到一定比例的人源细胞。结论:胎猪或新生猪能耐受一定量的人源抗原的植入,建立人/猪造血嵌合体模型是可行的。This study was aimed to explore the feasibility of transplanting human cord blood stem cells （HSC） into pre-immune fetal and neonatal pigs, and to investigate the self-renewal of HSC in the recipient pigs. The fetus and neonate were manipulated in sterile separated room and human donor cells were injected into fetus via fetus muscle or umbilical vein （dissectted womb） or into neonate via umbilical vein before cutting it. Human CD45^＋ cells s were detected by labeling with human anti-CD45 antibody and analyzed by fluorescence activated cell sorting （FACS）. The results showed that tested pigs developed as well as control and a definite proportion of human cells existed in peripheral blood of chimeric pig on day 60 after transplantation. In conclusion, the fetus and neonate pigs can tolerate a definite proportion of human antigens, and to establish the human/pig model of hematopoietic chimerism is possible.

关 键 词：脐血造血干细胞 异种移植 人/猪造血嵌合体 

分 类 号：R457.7[医药卫生—治疗学]