运用微卫星DNA标记分析我国野生鹌鹑遗传多样性  

Study on Genetic Diversity of Wild Quail in China with Microsatellite DNA Markers

作　　者：常国斌[1] 常洪[1] 刘向萍[1] 王惠影[1] 徐伟[1] 赵文明[1] 王庆华[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《Acta Genetica Sinica》2005年第8期795-803,共9页

基　　金：国家自然科学基金资助项目(编号:30070550)~~

摘　　要：运用微卫星DNA标记对我国境内分布的两种野生鹌鹑(野生日本鸣鹑、野生普通鹌鹑)和家鹑群体的遗传多样性进行分析。通过计算反映群体变异的多态信息含量(PIC)、固定指数、平均杂合度、基因分化系数等相关指标,结果表明:野生普通鹌鹑群体遗传多样性更为丰富,每个座位平均检测出4.67个等位基因,群体多态信息含量和平均杂合度亦为最高,分别为0.5732和0.6621;家鹑最低,分别为0.5467和0.5933;野生日本鸣鹑介于两者之间;在3个鹌鹑群体中,野生日本鸣鹑与家鹑群体遗传多样性差异程度较小。以标准遗传距离为基础的模糊聚类分析发现:家鹑与野生日本鸣鹑群体模糊等价矩阵系数为0.937,而与野生普通鹌鹑的系数为0.738,这也表明家鹑与野生日本鸣鹑有更近的亲缘关系,进一步从分子水平上证实家鹑起源于野生日本鸣鹑。Genetic diversity of domestic quail and two wild quail species distributed in China,wild Japanese quail and wild common quail,was studied by using microsatellite DNA markers. According to the comparison of corresponding genetic index in the three quail populations, such as polymorphism information content（PIC）,mean heterozygosity（H） and fixation index etc,wild common quail possessed rich genetic diversity of 4.67 alleles per locus. Its value of PIC and H were the highest,0. 5732 and 0.6621, respectively. Meanwhile, domestic quail had the lowest value,0. 5467 and 0. 5933, respectively. Wild Japanese quail had little difference in genetic diversity with domestic quail, in addition ,from analyses of fuzzy cluster based on standard genetic distance ,the similarity relation matrix coefficients between wild Japanese quail and domestic quail was 0. 937, and that between wild common quail and domestic quail was 0.783. All these results showed that wild Japanese quail was closer to the domestic quail in phylogenetic relationship than wild common quail. Mese results at the molecular level further proved the thesis that domestic quail is originated from wild Japanese quail.

关 键 词：微卫星 野生鹌鹁 遗传多样性 亲缘关系 DNA标记 

分 类 号：Q953.3[生物学—动物学]