免疫印迹法检测可提取核抗原多肽抗体的评价  

Estimate of ENA Polypeptide Autoantibody Detection Using I mmunoblotting Technique

作　　者：朱钰钰[1] 甘小丹[2] 

机构地区：[1]潮州市中心医院检验科,广东潮州521000 [2]北京协和医院风湿免疫实验室,北京100730

出　　处：《现代检验医学杂志》2005年第4期26-27,共2页Journal of Modern Laboratory Medicine

摘　　要：目的评价免疫印迹法(IB)检测可提取核抗原(ENA)多肽抗体的性能。方法以检测抗ENA抗体中的抗U1RNP抗体为研究对象,用免疫印迹法(IB)和免疫双扩散法(ID)分别进行检测,以高特异的ID法检测结果为对照,统计IB法的符合率。结果用IB法检测抗U1RNP抗体有7种不同的蛋白质排列组合,其中以73,32,17.5kDa三条蛋白带同时出现与ID法的符合率最高(84.1%),而32kDa的符合率最低(30.7%),这两组的差异最显著(P<0.01)。结论单独采用IB法检测抗ENA抗体尚欠可靠,须结合其它方法,取长补短,提高ENA抗体检测的敏感性、特异性。Objective To estimate the capability of immunoblotting（IB） detection of extractable nuclear antigen （ENA） polypeptide autoantibody. Methods Detection of anti-U1RNP antibodies in ENA autoantibody as study object,detection was carried out both in immunoblotting technique （IB） and immunodiffusion （ID）. The very specific ID detection result was compared. The coincidence rate of IB was counted. Results There were seven different protein combinations with immunoblotting detection U1RNP antibodies. Protein zones of 73 kDa,32 kDa and 17.5 kDa appeared simultanelty,and the coincidence rate （84.1%） with ID was the maximun. While the 32 kDa one with ID was the minimum The defference between these groups was the most obvious （P〈0. 01）. Conclusion It is lack of trustiness to detect ENA autoantibody by using IB singly. To enhance the indexes of sensitivity and specific,it is essential be combined with other methods.

关 键 词：免疫印迹 可提取核抗原多肽抗体 免疫双扩散 抗U1RNP抗体 

分 类 号：R446.6[医药卫生—诊断学]