吸烟对呼吸道上皮损伤机制的研究  

Study on damage of airway epithelium cell and its mechanism in smoking mice

作　　者：刘志艳[1] 隋少峰[2] 王志富[1] 高鹏[1] 牟坤[1] 吴人亮[3] 

机构地区：[1]山东大学齐鲁医院病理科,济南250014 [2]山东省疾病预防控制中心 [3]华中科技大学

出　　处：《中国公共卫生》2005年第8期897-899,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目(39870306)

摘　　要：目的研究吸烟小鼠不同阶段气道上皮的形态学变化以及上皮γ-连环素(γ-catenin,γ-cat)的表达,探讨γ-cat在吸烟对呼吸道上皮损伤过程中的作用。方法动物实验按时间将段分成5组:S1M、S2M、S4M、S5M、S6M及正常对照组(SC)。以常规苏木素-伊红(HE)染色光镜观察其形态学变化;以免疫组织化学技术研究γ-cat的表达;以原位杂交技术研究γ-catmRNA的表达;以图像分析技术随机检测15个细支气管上皮细胞表达的平均吸光度值。结果随吸烟时间延长,小鼠气道上皮细胞形态依次呈损伤、修复、过度增殖的形态学改变;除S5M组γ-cat染色的平均吸光度值高于SC组外,吸烟组小鼠气道上皮细胞γ-cat染色的平均吸光度值均低于SC组;SC组小气道上皮增殖细胞核抗原(proliferingcellnuclearantigen,PCNA)阳性细胞较少(P<0·001)。S1M组小气道上皮PCNA阳性细胞虽有增加但不明显。其余各吸烟组小鼠小气道上皮PCNA阳性细胞率均明显增高(P<0·01)。结论香烟烟雾对气道上皮存在损伤作用;γ-cat在呼吸道上皮细胞损伤与修复中发挥作用。Objective To investigate the changes of morphology and the effect of γ—catenin（γ—cat） in damage of respiratory epithelium in different stage of smoking mice. Methods Observation under microscope after HE staining was used to study the morphological changes of airway epithelium of different stages（ smoking after 1 month/S1 M, 2 months/S2 M, 4 months/S4M, 5 months/S5M, 6 months/S6M and the control/SC）in smoking mice. The expression of γ—cat was determined by immunochemical stain, that of γ-cat mRNA was estimated by in situ hybridization, the mean A value was determined by image analysis of randomly selected 15 thin branch in smoking mice. Results Morphology of airway epithelium in smoking mice changed from injury to repair, proliferation, and over proliferation; the protein and mRNA levels of γ-cat of each stage were significantly lower than those of the control except the protein level of the S 5M（ P〈0.001 ） ;expression of prolifering cell nuclear antigen of airway epithelium cell in smoking mice was higher than that of the control, S 2M group was the highest（P〈0.01）. Conclusion Smoke inhalation injuried the airway epithelium;γ—cat was wavy changed and functions in the course of the damage and repair of the airway epithelium.

关 键 词：Γ-连环素 损伤 修复 气道上皮 吸烟 

分 类 号：R994.6[医药卫生—毒理学]