德国小蠊DDVP抗性与相关酶活性的关系  

Relationship between DDVP resistance of German cockroaches and related enzyme

作　　者：郭丽[1] 沈孝兵[1] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,南京210009

出　　处：《中国公共卫生》2005年第8期938-939,共2页Chinese Journal of Public Health

基　　金：铁道部站车卫生科研基金资助项目(J99Z217)

摘　　要：目的探讨乙酰胆碱酯梅(AChE)、α-乙酸萘酯酶(1-NA)、谷胱甘肽S-转移酶(GSTs)活性与德国小蠊敌敌畏(DDVP)抗性水平的关系。方法采用分光光度法和微量板酶动力学法分别测定不同DDVP抗性水平德国小蠊的AChE、1-NA和GSTs酶活性,应用SAS软件进行统计分析。结果随着德国小蠊DDVP抗性水平的增加,1-NA及GSTs酶活性逐渐性逐渐升高,AChE活性仰制率和1-NA酶活性在德国小蠊抗性系数为2·8及以下时与敏感株相比,差异均有统计学意义P<0·05。GSTs酶活性在抗性系数2·8及以下与敏感株相比,差异无统计学意义。结论AChE的抑制率和1-NA酶活性可作为德国小蠊对DDVP抗性的生物化学标记。Objective To study the relationship between the activities of acetycholine esterase, 1-NA, glutathione Stransferase and DDVP resistance of German cockroaches（ Blattella germanica）. Methods To measure the activities of enzymes above-mentioned in different DDVP-resistant stains of German cockroaches by using visible spectrophotometry and kinetic microplate methods, and to carry through the statistical, analysis by SAS software. Results With DDvp-resistant levels＇ increased in Germand cockroaches, ACHE＇s inhibition ratio, 1-NA and glutathone S-transferase enhancd gradually. The distinction was remarkabkle on inhibition ratio of acetycholine esterase＇s activity and 1-NA＇s activity among resistant strains and sensitive strains when resistant coefficient equal or less than 2.8. Nevertheless, the distinction was not remarkable for the activity of glutathione s-transferase. Conclusion AChE＇s inhibition ratio and 1-NA＇s activity are proved to some values as biomarkers of German cockroaches＇ DDVP-resistance.

关 键 词：德国小蠊 乙酰胆碱酯酶 α-乙醛萘酯酶 谷胱甘肽S-转移酶 抗药性 敌敌畏 

分 类 号：R184.39[医药卫生—流行病学]