3Gyγ射线对不同肿瘤细胞细胞周期进程的影响  

Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays

作　　者：夏景光[1] 李文建[1] 魏巍[1] 赵靖[1] 金晓东[1] 高清祥[2] 

机构地区：[1]中国科学院近代物理研究所 [2]兰州大学生命科学学院,兰州730000

出　　处：《核技术》2005年第8期621-624,共4页Nuclear Techniques

基　　金：国家自然科学基金重点项目(10335050);国家重大基础研究前期研究专项项目(2003CCB00200)资助

摘　　要：本实验旨在研究电离辐射对不同肿瘤细胞细胞周期进程的影响,为肿瘤的放射治疗提供科学依据。以3Gyγ射线照射指数生长期的肿瘤细胞,用流式细胞术测定细胞DNA含量并确定细胞周期各时相的细胞百分比。实验结果显示,γ射线照射后,SMMC-7721,hepG2和HO-8910细胞的G2/M期发生明显阻滞,且都在辐射后12h累积达到最大值,但S期只发生短暂的延迟,即辐射后6h明显累积,之后下降至接近对照水平或低于对照;Hela细胞的G2/M期和S期均发生显著阻滞。实验结果说明,电离辐射后,不同肿瘤细胞的G2/M期和S期检查点的激活和维持是有差异的。Exponentially growing human tumor cell lines were γ-irradiated with a dose of 3Gy. The DNA content and percentage of each cell-cycle phase were obtained with flow cytometry. Results indicated that for SMMC-7721,hepG2 and HO-8910 cells, G2/M accumulation was prominently induced by irradiation and the maximal accumulation appeared at 12h after treatment, and that S phase just displayed temporal accumulation, i.e. S phase cells accumulated at 6h after irradiation, and thereafter decreased close to or below the level of un-irradiated cells,and that for Hela cells both G2/M and S phase accumulation were pronouncedly triggered by irradiation. It is suggested that there remain differences in activation and maintenance of cell cycle checkpoints after irradiation among these four tumor cell lines.

关 键 词：电离辐射 肿瘤细胞 细胞周期 细胞周期检查点 

分 类 号：Q691[生物学—生物物理学]