人卵泡液中白血病抑制因子与卵泡发育及性激素水平的关系  

The Relationship between the Level of Leukaemia Inhibitory Factor in Human Follicular Fluid and Follicle Development and Sex Hormones Level

作　　者：吕俊[1] 周从容[2] 李荣荣[2] 赵淑云[2] 罗锦[2] 

机构地区：[1]山东省济南市妇幼保健院,250001 [2]贵阳医学院附属医院,550004

出　　处：《贵州医药》2005年第8期683-685,共3页Guizhou Medical Journal

摘　　要：目的检测接受体外受精-胚胎移植(IVF-ET)的不孕患者取卵日卵泡液中白血病抑制因子(LIF)水平,探讨其与卵母细胞成熟度及甾体激素水平关系。方法采用ELISA法检测61例IVF-ET惠者取卵日血清及卵泡液中的LIF水平、采用放射免疫法(RIA)检测卵泡液中雌二醇(E2)、孕酮(P)、睾酮(T)水平,并记录每份卵泡液中的卵母细胞成熟度。结果(1)卵泡液中的LIF水平为(403.45±128.15)pg/ml,显著高于血清中LIF水平[(3.52±1.54)pg/ml)](P<0.001)。(2)成熟卵细胞卵泡液中LIF水平为(529.5±218.17)pg/ml,显著高于不成熟组[(194.70±92.65)pg/ml] (P<0.001)及过熟组[(321.42±131.35)pg/ml](P<0.05)。(3)卵泡液中LIF水平与P成正相关(γ=0.343,P=0.007),与E2、T无相关性。结论卵泡液中高水平的LIF可能为卵巢源性,其与卵母细胞的成熟度有密切关系,并且与卵泡液中的甾体激素有一定的相关性。Objective To detect the concentration of leukaemia inhibitory factor（LIF） in human follicular fluid（FF） in vitro fertilization and embryo transfer（IVF-ET） ,and to evaluate its relationship to oocyte maturity and sex hormones levels. Methods LIF concentrations in FF and serum samples obtaining from 61 patients undergoing IVF-ET were measured with ELISA. The levels of estradiol（E2 ）,progesterone （P）,testosterone （T） were determined by radioimmunoassay. The maturity of each oocyte was identified. Results The LIF mean concentration in FF[（403.45±128. 15） pg/ml] was significantly higher than that in serum[（3.52±1.54） pg/ml] （P〈0. 001）. The LIF mean concentration in FF with mature oocytes [（529.56±218. 17） pg/ml] was significantly higher than those with premature oocytes [（194.70±92. 65） pg/ml] （P〈0. 001） and overmature oocytes [（321. 42±131. 35） pg/ml] （P〈0. 05）. In FF, the concentration of LIF was positively correlated to P level （γ=0. 343, P〈0. 05）. No positive correlation was found between the level of LIF and the level of E2 and T in FF.Conclusion Higher concentration of LIF exist in FF and it may have a physiological role in promoting oocyte development and maturity.

关 键 词：白血病抑制因子 卵泡液 受精 体外 甾体激素 

分 类 号：R458.7[医药卫生—治疗学] R711.6[医药卫生—临床医学]