黄海伪虎鲸mtDNA控制区序列的测定和初步分析  

An Initial Study on the Mitochondrial DNA Control Region Sequences of False Killer Whales (Pseudorca crassidens)

作　　者：张婷[1,2] 杨光 周开亚[1,2] 魏辅文[3] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物资源技术重点实验室 [2]南京师范大学生命科学学院遗传资源研究所,南京210097 [3]中国科学院动物研究所

出　　处：《兽类学报》2005年第3期219-223,共5页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(30270212和300710116);国家杰出青年科学基金资助项目(30125006);国家"211工程""十五"建设项目;江苏省"青蓝工程"中青年学术带头人科研基金资金项目

摘　　要：测定了黄海7头伪虎鲸mtDNA控制区5’端533bp的序列,共发现54个变异位点,定义了4种单元型。最大似然法和邻接法的系统树均把单元型聚为2分支。两支之间没有共享单元型且无姊妹关系。分支间的核苷酸序列差异显著地超过了其它海豚类种间的序列差异水平,提示传统意义上的伪虎鲸可能包括了具有显著遗传差别的不同物种,迫切需要开展进一步的研究。Five hundred and thirty three base pairs （bp） of the mitochondrial control region of seven false killer whales （ Pseudorcu crassidens） from the Yellow Sea in China were sequenced.Fifty-four variable sites were determined and four haplotypes were identifled. Phylogenetic analyses using maximum likelihood and neighbor-joining algorithms divided the haplotypes of false killer whales into two clades showing no sister relationship with each other. No shared haplotypes were found between the two clades. The average nucleotide divergence between the two clades is approximately 7.61%, much higher than those, between Turisops species （5.58%） and between Delphinus species （1.09% ）,which suggested that the two clades represent respective species. Further study is urgently needed.

关 键 词：伪虎鲸 黄海水域 MTDNA控制区 物种分化 

分 类 号：Q754[生物学—分子生物学]