广东省韶关地区戊型肝炎病毒基因型分析  

Phylogenetic analysis of hepatitis E virus (HEV) in fecal samples from pigs and in serum from human in Shaoguan ,Guangdong province

作　　者：柯昌文[1] 黄一伟[2] 邓俊兴 李晖[1] 李天成 唐建华[3] 邹丽容[1] 王洪敏[1] 张勤奋[2] 林锦炎[1] 武田直和 张景强[2] 

机构地区：[1]广东省疾病预防控制中心,广东广州510300 [2]中山大学 [3]韶关市疾病预防控制中心 [4]日本国立感染症研究所

出　　处：《华南预防医学》2005年第4期1-4,11,共5页South China Journal of Preventive Medicine

基　　金：日美医学协力研究会基金资助

摘　　要：目的了解韶关地区流行的戊型肝炎病毒基因型,并探讨戊型肝炎是否属于人兽共患病.方法通过同源比对HEV四种基因型的戊型肝炎病毒序列,选取其保守序列部分设计两对巢式聚合酶链式反应的简并引物,建立一种可同时扩增戊型肝炎四个基因型病毒RNA的PCR快速检测方法.在广东省韶关地区采集猪粪便标本和非甲非乙型肝炎病人血清,提取病毒RNA,然后进行PCR检测,阳性时对扩增片段进行基因序列测定,并进行基因型分析.结果 RT-nPCR检测结果显示99份猪粪便标本中有6份HEV RNA阳性,7份病人血清样本中1份阳性,序列分析显示全部为HEV基因IV型,4份猪标本中的HEV基因序列与GenBank中人HEV的基因序列最相似,同源性为88%～94%,且其中1份猪标本中的HEV与1份病人标本中HEV的序列同源性高达91.5%.结论广东韶关地区HEV流行株为基因IV型,而且猪HEV基因序列与人HEV之间有着高度的相似性,结果证实戊型肝炎是一种人兽共患传染病.Objective To investigate the genotyping of HEV in Shaoguan, Guangdong province. Methods Two pairs of consensus primers were designed according to the conserved sequence region from four - genotype HEV strains for nested PCR. HEV RNA in pig fecal sample and in serum from patient with non - A, non - B hepatitis was detected by nested PCR. Positive PCR product was sequenced and analyzed phylogenetically with neighbor - joining method. Results HEV RNA was detected in 6 of the 99 pig fecal samples and 1 of the 7 patient sera. Sequence analysis show that all of the positive isolates were genotype Ⅳ. Conclusion These findings suggest that HEV genotype Ⅳ is the endemic strain in Shaoguan and hepatitis E is a zoonosis.

关 键 词：肝炎病毒 戊型 聚合酶链反应 基因 病毒 

分 类 号：R512.6[医药卫生—内科学]