Preparation of Nanosized CoHCF Modified Electrode and Its Application to Electroanalysis of Hemoglobin  

作　　者：刘梅川 李平 张莉 鲜跃仲 丁红春 张成林 张芬芬 金利通 

机构地区：[1]Department of Chemistry, East China Normal University, Shanghai 200062, China

出　　处：《Chinese Journal of Chemistry》2005年第8期983-989,共7页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of C＇hina（No. 20305007）, Doctoral Foundation of China University of Ministry of Education （No. 20030269014） and Shanghai Rising-Star Program （No. 04QMX 1421）.

摘　　要：A cobalt hexacyanoferrate （CoHCF） nanoparticle （size ca. 60 nm） chemically modified electrode （CME） was fabricated and the electrochemical behavior of hemoglobin （Hb） at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon electrode （GCE） and a general CoHCF CME electrodeposited in a traditional manner, the present nanosized CoHCF CME performed efficiently electrocatalytic reduction for Hb with relatively high sensitivity, stability, and longlife, Combined with liquid chromatography （LC）, the nanosized CoHCF CME was used as the electrochemical detector of Hb in the established flow injection analysis-electrochemical determination （FIA-ECD） system. The peak current was a linear function of concentrations in the range from 2.5×10^-8 to 5.0×10^-6mol/L for Hb, with detection limit of 1.4×10^-8 mol/L. The FIA-ECD system has been successfully applied to assess the Hb content of clinic blood samples with advantages of sensitiveness, speediness, easy control and small sample-consumption.A cobalt hexacyanoferrate （CoHCF） nanoparticle （size ca. 60 nm） chemically modified electrode （CME） was fabricated and the electrochemical behavior of hemoglobin （Hb） at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon electrode （GCE） and a general CoHCF CME electrodeposited in a traditional manner, the present nanosized CoHCF CME performed efficiently electrocatalytic reduction for Hb with relatively high sensitivity, stability, and longlife, Combined with liquid chromatography （LC）, the nanosized CoHCF CME was used as the electrochemical detector of Hb in the established flow injection analysis-electrochemical determination （FIA-ECD） system. The peak current was a linear function of concentrations in the range from 2.5×10^-8 to 5.0×10^-6mol/L for Hb, with detection limit of 1.4×10^-8 mol/L. The FIA-ECD system has been successfully applied to assess the Hb content of clinic blood samples with advantages of sensitiveness, speediness, easy control and small sample-consumption.

关 键 词：nanosized cobalt hexacyanoferrate chemically modified electrode HEMOGLOBIN direct electrochemical determination flow injection analysis-electrochemical determination clinic blood 

分 类 号：R446.11[医药卫生—诊断学] O646.5[医药卫生—临床医学]