消渴颗粒剂对大鼠系膜细胞TGF-β_1 mRNA表达的影响  

Influence of Xiaoke Granules on expression of TGF-β_1 mRNA in rat mesangial cells

作　　者：王谦[1] 周彩虹[2] 龚慕辛[3] 于玫[3] 赵晖[3] 黄启福[1] 李克玲[1] 蒋玉凤[1] 贾德贤[1] 

机构地区：[1]北京中医药大学,北京100029 [2]中国医学科学院肿瘤医院医务处,北京100021 [3]首都医科大学中医药学院,北京100013

出　　处：《北京中医药大学学报》2005年第4期36-38,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京市教育委员会科技发展计划项目(No.00KJ-074);北京市中医药管理局资助课题(2000年)

摘　　要：目的探讨消渴颗粒剂治疗糖尿病肾病的机制。方法用高浓度葡萄糖刺激系膜细胞,加入中药含药血清后,采用逆转录多聚酶链反应(reversetranscriptasepolymerasechainreaction,RT-PCR)方法观察中药对高糖刺激后系膜细胞TGF-β1mRNA表达的影响。结果高浓度葡萄糖可刺激系膜细胞TGF-β1mRNA表达,而中药可抑制TGF-β1mRNA的过度表达。结论消渴颗粒剂可抑制高糖引起的TGF-β1mRNA过度表达,这可能是其防治糖尿病肾病的关键。Objective To investigate the therapeutic mechanism of Xiaoke Granules for diabetic nephropathy. Method Cultured rat mesangial cells （MCs） were exposed to the high-level glucose, and then the serum with Xiaoke Granules was added. The effect of Xiaoke Granules on the expression of tumor growth factorβ1（TGF-β1） mRNA in MCs was observed through reverse transcriptase polymerase chain reaction （RT-PCR）. Result The high-level glucose irritated the expression of TGF-β1 mRNA, while Xiaoke Granules inhibited the excessive expression of TGF-β1 mRNA in MCs. Conclusion Xiaoke Granules can inhibit the excessive expression of TGF-βS1 mRNA, which maybe its key effect for treating diabetic nephropathy.

关 键 词：消渴颗粒剂 大鼠 TGF-Β1 基因表达 

分 类 号：R285.5[医药卫生—中药学]