薯蓣皂苷元对人胃低分化粘液腺癌细胞作用的研究  

Effect of diosgenin on poor differentiated adenocarcinoma cells of human stomach

作　　者：宋宇[1] 何忠梅[1] 李晶华[2] 霍锐[2] 刘大鹏[1] 王本祥[1] 

机构地区：[1]吉林天药科技有限公司国家规范化中药药理实验室,吉林130012 [2]吉林大学,吉林130021

出　　处：《北京中医药大学学报》2005年第4期42-44,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局新药开发专项课题

摘　　要：目的观察薯蓣皂苷元(Diosgenin,Dio)对人胃低分化粘液腺癌(MGC-803)细胞的作用,并初步探讨其作用机制。方法MTT法测Dio对MGC-803细胞的抑制作用;考察Dio对MGC-803细胞分裂和集落形成能力的影响;采用3H-TdR掺入法检测Dio对MGC-803细胞DNA合成的影响。结果一定浓度范围内Dio对MGC-803细胞的增殖有剂量、时间依赖性抑制作用;Dio明显抑制MGC-803细胞分裂和集落形成,半数抑制浓度(IC50)为13.17mg/L,Dio能显著抑制MGC-803细胞DNA合成。结论Dio对MGC-803细胞的抑制作用与抑制该细胞DNA合成有关。Objective To observe the effect of diosgenin （Dio） on poor differentiated adenocarcinoma （MGC-803） cells of human stomach and discuss the mechanism. Methods MTr assay was used to determine the inhibitory effect of Dio on MGC-803 cells, and to observe the influence of Dio on the ability of cell cleavage and colony formation. The method of 3^H-TdR was used to detect the influence of Dio on DNA synthesis of MGC-803 cells. Result It was found that Dio inhibited the growth of MGC-803 ceils in a dose-time dependent manner at a certain range of concentration, and inhibited obviously the cell cleavage and colony formation. IC50 was 13.17 μg/mL. Dio inhibited obviously DNA synthesis of MGC-803 cells. Conclusion The inhibitory effect of Dio on MGC-803 cells is related to its inhibitory effect on DNA synthesis.

关 键 词：薯蓣皂苷元 抗肿瘤作用 集落形成 ^3H-TDR掺入 

分 类 号：R285.5[医药卫生—中药学]