脾虚大鼠结肠电及动力基本功能单位病理改变研究  

Pathological changes of colonic electromuscular and dynamic basic functional unit in rats with syndrome of spleen deficiency

作　　者：李卫东[1] 张书征[2] 连至诚[2] 

机构地区：[1]广东药学院基础学院,广东510006 [2]广州中医药大学脾胃研究所,广东510006

出　　处：《北京中医药大学学报》2005年第4期49-51,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：广东药学院科研基金项目(No.2005KJ066)

摘　　要：目的研究长期应用大黄对结肠肌电皮动力基本功能单位的影响,探讨Cajal间质细胞(ICC)在脾虚大鼠结肠电中的作用。方法50只大鼠随机分为正常对照组、大黄组,3个月后测定结肠慢波频率及振幅,用碘化锌-锇酸法(ZIO)观察肌间神经丛及ICC变化,透射电镜观察结肠动力基本功能单位即壁内神经-Cajal间质细胞-平滑肌细胞(ENS-ICC-SMC)的病理改变。结果长期服用大黄所致脾虚大鼠,结肠慢波频率及振幅明显下降;结肠电节律失常;慢波频率变异系数(CV)及异常节律指数(ARI)显著增加;肌间丛神经纤维分布不均匀,突起连接杂乱,视野ICC稀少;电镜下见肌间神经轴突空化,ICC细胞样变性。结论长期服用大黄导致脾虚大鼠结肠慢波频率减慢,其机制可能为结肠动力基本功能单位的病理改变。Objective To study the influences of Rheum palmatum L. （administrated for a long time） and the interstitial Cajal cell （ICC） on colonic electromuscular in rats with spleen deficiency. Methods Fifty rats were randomly divided into the control group and the group administrated Rheum palmatum L. After 3 months the slow wave frequency and amplitude of colon were detected. The changes of ICC were observed by using ZIO method and the pathological changes of dynamic basic functional unit （DBFU, i.e. ENS-ICC-SMC） were observed with transmission electron microscope （TEM）. Results In rats with spleen deficiency induced by long-time administration of Rheum palmatum L. the slow wave frequency and amplitude of colon decreased. The electric rhythm of colon was abnormal. The coefficient of variation（CV） of slow wave frequency and abnormal rhythm index （ARI） increased obviously. The neurofibers of intennuscular neuroplexus were poor distributed, the connection was disorder and ICC was few in TEM vision. The vacuolar degeneration of intennusclar nervous axon and degeneration of ICC were observed by TEM. Conclusion The long-time administration of Rheum palmatum L. can induce the decrease of slow wave frequency in rats with spleen deficiency. The mechanism may be related to the pathological changes of DBFU.

关 键 词：脾虚 肠神经系统 CAJAL间质细胞 

分 类 号：R285.5[医药卫生—中药学]