天灸对肺癌小鼠肿瘤转移抑制基因mRNA表达影响的实验研究  

Influence of natural moxibustion on mRNA expression of nm23-1 gene in mice with lung cancer

作　　者：李南玲[1] 赵立岩[1] 李学武[1] 丁良伊[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《北京中医药大学学报》2005年第4期90-92,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：教育部高等学校博士学科点专项科研基金资助课题(No.20020026012)

摘　　要：目的研究天灸对Lewis肺癌化疗小鼠nm23-1基因的mRNA表达的影响。方法用实时荧光定量逆转录-多聚酶链反应(RT-PCR)技术检测nm23-1的mRNA表达。结果单纯化疗组nm23-1的mRNA表达与对照组比较,差异无统计学意义;天灸和天灸配合化疗组显著促进Lewis肺癌小鼠nm23-1的mRNA表达,且天灸配合化疗组对Lewis肺癌的生长有明显的抑制作用。结论提高nm23-1基因的mRNA表达,可能是天灸增强化疗药物的抗癌机制之一。Objective To study the influence of natural moxibustion （NM） on mRNA expression of nm23-1 gene in mice with lung cancer. Method The mRNA expression of nm23-1 gene was detected by the technique of real-time fluorescence quantitative reverse transcriptase-polymerase chain reaction （RT- PCR）. Result There was no significant difference in the chemiotherapy group compared with the control group in mRNA expression of nm23-1 gene （ P 〉 0.05）. The mRNA expression of nm23-1 gene was obviously improved and Lewis lung cancer was obviously inhibited in the NM group and the group of NM combined with chemiotherapy. Conclusion One of the anti-cancer mechanisms of NM may be that it can improve mRNA expression of nm23-1 gene through helping chemiotherapy.

关 键 词：天灸 LEWIS肺癌 肿瘤转移抑制基因 

分 类 号：R285.5[医药卫生—中药学]