多聚乙醇酸-羟基磷灰石复合体为支架的骨髓基质细胞修复兔关节软骨缺损  

Repair of articular cartilage defects by bone marrow derived mesenchymal cells combined with hydroxyapatites-polyglycolic acid composite

作　　者：周晓中[1] 张文智[2] 吕维佳[2] 董启榕[1] 陈振腾[3] 梁智仁[2] 

机构地区：[1]苏州大学附属第二医院骨科,215004 [2]香港大学骨科学系 [3]香港大学生化学系

出　　处：《中华创伤杂志》2005年第8期617-620,共4页Chinese Journal of Trauma

摘　　要：目的研究以多聚乙醇酸(polyglycolicacid,PGA)、羟基磷灰石(hydroxyapatites,HA)复合体为支架的骨髓基质细胞(bonemarrowderivedmesenchymalcells,BMSCs)修复兔膝关节软骨缺损修复效果。方法自体BMSCs分别种植于PGA和HA支架后共同培养,生物胶粘连两种支架-细胞复合物形成BMSCs-PGA-HA复合体,植入兔膝关节软骨缺损模型。术后20,32周处死动物,标本行组织学检查及Ⅱ型胶原免疫组化染色。结果BMSCs-PGA-HA复合体植入后形成稳定的透明软骨样修复组织及软骨下骨,该组织与周围正常软骨及软骨下骨融合良好,32周后软骨标本无明显退变。结论以BMSCs为种子细胞、更符合缺损处生理结构的PGA-HA为支架的复合体,可修复关节软骨缺损,中长期效果值得肯定。Objective To observe the effect of implantation of bone marrow derived mesenchyreal cells （BMSCs） combined with polyglycolic - acid-hydroxyapatites （PGA-HA） composites in repairing articular cartilage defects. Methods Culture-expanded rabbits＇ BMSCs were seeded onto porous PGA and HA scaffolds respectively. The cell-adhered PGA and HA were joined together to form a composite that was then implanted into articular cartilage defects. The rabbits were then sacrificed at 20 and 32 weeks respectively. The obtained specimens were examined histologically and stained immunohistochemically for type I1 collagen. Results Specimens harvested from BMSCs-PGA-HA composite implantation demonstrated hyaline cartilage and a complete subchondral bone formation. The newly formed tissue with subchondral bone bad perfect fusion with surrounding normal cartilage. A relative long-term follow up showed no progressive degeneration sign of newly formed tissues at the 32nd week. Conclusions BMSCs-PGA-HA composites facilitate the formation of hyaline-like cartilage and the integration between subchondral bone and surrounding cartilage. Therefore, BMSCs-PGA-HA composites can repair articular cartilage defects and have a better long-term result.

关 键 词：多聚乙醇酸 羟基磷灰石 复合体 支架 骨髓基质细胞 修复 关节软骨缺损 

分 类 号：R687[医药卫生—骨科学]