从母婴分离的念珠菌DNA分型研究  

A DNA Genotyping Study of Candida Isolates from Mothers and Their Neonates

作　　者：席丽艳[1] 福岛和贵[2] 滝泽香代子[2] 鲁长明[1] 岑丽卿 西村和子[2] 

机构地区：[1]中山大学附属第二医院皮肤性病科,广州市510120 [2]日本千叶大学真菌医学研究中心 [3]佛山市妇婴医院皮肤科

出　　处：《中华皮肤科杂志》2005年第8期478-481,共4页Chinese Journal of Dermatology

基　　金：广东省佛山市重点科技攻关基金资助项目[(2001)0108010A]

摘　　要：目的探讨念珠菌在母婴间垂直传播的可能途径。方法采用分子生物学方法,对11对分离于分娩前母亲阴道分泌物及其新生儿口腔并经常规形态学和生化学鉴定为同种念珠菌的母婴分离株,再次进行大亚基rDNA的D1/D2区域DNA序列鉴定。采用电泳核型和随机扩增DNA多态性进行分型研究,以确定是否为同一来源的菌株。结果11对22株菌,其中8对母婴分离株的16株菌的DNA序列经DDBJGeneBank(登录号U45776)搜索与白念珠菌参考株Cal_Y12983具有100%的同源序列,被确认为白念珠菌;另2对的4株菌和1对的2株菌的DNA序列经DDBJGeneBank搜索与Cgra_Y65(U44808)和C.kruseiY-5396(U76347)具有100%的同源序列,分别被确认为光滑念珠菌和克柔念珠菌。电泳核型分析,每一对白念珠菌和克柔念珠菌的母婴分离株都具有完全相同的谱带。每一对光滑念珠菌的母婴分离株尽管产生1、2条不同谱带,但仍被认为是来源于同一个体,因为主要谱带是相同的。RAPDDNA带谱分析,对母婴分离株除1对2株菌的DNA谱带略有不同外,其余10对皆具有同样的DNA谱带。结论1111株婴儿株与其母亲株分子生物学特征完全相同,可认为是经母亲产道垂直传播所致。Objectives To identify the route and time of transmission by Candida species from mothers＇ vagina to their neonates＇ mouth. Methods Specimens for fungal cultures were obtained from vaginal discharge of mothers just before delivery and also from the mouth of their offspring just after birth. Eleven mother-infant pairs were investigated. Candida speeies was identified based on morphology, biochemical analysis, and sequencing of D1/D2 domain of the large subunit of ribosomal DNA （LSUrDNA）. Electrophoretic karyotyping（EK） and random amplified polymorphic DNA analysis （RAPD） were performed to search for DNA homology. Results Candida isolates （16 strains） from 8 mother-infant pairs were identified as Candida albicans by 100% homology of their D1/D2 sequences with reference strain C. albicans Y-12983 （GenBank access No. U45776）. Similarly, 4 strains from two mother-infant pairs and 2 isolates from the other pair were identified as Candida glabrata and Candida krusei, respectively, by 100% homology in sequences alignment of the domains with reference strains, C. gladrata Y-65（U44808） and C. krusei Y-5396 （U76347）. The same EK profiles were found for each C. albicans or C. krusei strain pair from both mother and her neonate. Although different EK bands with various molecular size were generated for eacb C. glabrata isolate pair, they were still considered to be homologous based on the fact that main EK bands were identical. Each isolate pair from mother and her infant presented almost the same RAPD profile, except for one pair, isolates F7n and F7m, which showed minor diverse DNA bands. Conclusion Eleven Candida isolates from neonates have identical molecular eharaeteristics with their mother＇s isolates. Vertical transmission may be the main pathway of Candida spp. from mothers to their neonates.

关 键 词：念珠菌属 疾病传播 垂直 念珠菌DNA 母婴分离 分型研究 随机扩增DNA多态性 母婴垂直传播 DNA序列 分子生物学方法 

分 类 号：R379[医药卫生—病原生物学]