马拉色菌临床株分类鉴定的研究  

Genotyping of Clinical Isolates of Malassezia from Patients with Tinea Versicolor by PCR-RFLP

作　　者：崔凡[1] 陶诗沁[2] 沈永年[1] 吕桂霞[1] 陈伟[1] 李筱芳[1] 胡素泉[1] 杨丽佳[2] 刘维达[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042 [2]无锡市第二人民医院皮肤科

出　　处：《中华皮肤科杂志》2005年第8期492-494,共3页Chinese Journal of Dermatology

摘　　要：目的用PCR-RFLP方法对马拉色菌临床株快速分类,并结合生化鉴定分析结果的可靠性。方法先用吐温试验和过氧化氢酶试验对74株分离自花斑癣的马拉色菌临床株和7株标准菌株进行分种,再用特异性引物扩增马拉色菌的28SrDNA,对扩增产物分别用Eco88Ⅰ、Bsp143Ⅱ和BshNⅠ进行RFLP分析。结果3种限制性内切酶成功地将限制、钝形和厚皮马拉色菌鉴定出来,并发现限制马拉色菌在基因同源性上与其他6种马拉色菌存在较大差异。此外通过RFLP法还纠正了生化分型的错误。结论PCR-RFLP法是一种有希望能够快速、准确对马拉色菌属进行种间分类的分子生物学技术。Objectives To develop a rapid genotyping method of clinical isolates of Malassezia from patients with tinea versicolor by PCR-RFLP, and to evaluate reliability of the approach as compared with biochemical classification. Methods Tween assimilation test and catalase reaction were carried out to identify 74 isolates of Malassezia species from patients with tinea versicolor anti 7 Malassezia reference strains. The sequence of 28S rDNA of Malassezia species was amplified by PCR, and then the product was analyzed by RFLP with Eco88Ⅰ, Bsp143Ⅱ and BshN Ⅰ , respectively. Results M. restricta, M. obtusa and Mpchydermatis were successfully identified hy three restriction endonucleases. M. restricta was found to be more diverse from the other 6 species in genetic homology. By comparison with PCR-RFLP technique, a possible mistake was discovered with biochemical method. Conclusion PCR-RFLP is a promising molecular biological technique, which could rapidly and correctly classify Malassezia species.

关 键 词：马拉色霉菌属 花斑癣 厚皮马拉色菌 分类鉴定 临床株 PCR-RFLP方法 限制性内切酶 PCR-RFLP法 分子生物学技术 RFLP分析 

分 类 号：R756[医药卫生—皮肤病学与性病学]