柱子型直接固定氯化血红素及其应用  

Immobilization of Hemin as the Columns and Their Application

作　　者：张小芬[1] 李任强[1] 徐思光[1] 熊永康[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《广东化工》2005年第7期16-18,共3页Guangdong Chemical Industry

基　　金：教育部留学回国人员科研基金(2002247)

摘　　要：用惰性载体Sepharose 4B、琼脂糖、微晶纤维素、人造沸石等分别直接固定氯化血红素,分别装柱后应用于痕量过氧化氢及血清葡萄糖浓度的检测,并比较它们的检测灵敏度。结果表明,以上几种载体经活化后都能很好地固定血红素,固定化的血红素装柱后保持稳定的催化过氧化物的活性,检测灵敏度高,血红素在几种载体上检测灵敏度的大小为:Sepharose 4B>琼脂糖>微晶纤维素>人造沸石。本法操作简单且成本低,柱子型直接固定氯化血红素可反复使用,能快速检测样品中的痕量过氧化氢及用于血清中葡萄糖含量的检测。Sepharose 4B, agarose, cellulose microcrystalllne and permutlt were respectively used as the vehicle to immobilize hemln to make the columns, which were applied to measure trace hydrogen peroxide and glucose concentration in serum, and the measurement sensitivity of these columns were examined. Results showed that hemin may be immobilized well by these activated vehicles. The hemin columns presented a stable activity of hemin and a high measurement sensitivity, but the measurement sensitivity of hemln was different in different vehicles as following： Sepharose 4B〉 agarose 〉 cellulose microcrystalline 〉 permutit. Manufacture of hemin column was cheap. The column may be used repeatedly and applied for quick examination of trace hydrogen peroxide or glucose concentration in serum, which was valuable in clinic and environmental chemistry.

关 键 词：惰性载体 氯化血红素固定 血红素反应柱 过氧化氢测定 葡萄糖浓度检测 氯化血红素 SEPHAROSE 应用 柱子 检测灵敏度 

分 类 号：TQ352.9[化学工程] Q554.6[生物学—生物化学]