自身免疫性甲状腺疾病血IL-1β与PBMC抗原的关系  

Study on the Relationship between IL-1β and PBMC Antigen in Autoimmune Thyroid Disease

作　　者：刘东方[1] 邓华聪[2] 许改平[1] 侯平 雄波 

机构地区：[1]重庆医科大学附二院内分泌科,400010 [2]重庆医科大学附一院内分泌科 [3]重庆壁山县人民医院

出　　处：《天津医药》2005年第8期476-478,共3页Tianjin Medical Journal

基　　金：重庆市卫生局青年基金项目(项目编号:2001-1-15)

摘　　要：目的:探讨自身免疫性甲状腺疾病(AITD)患者IL-1β水平与外周血单个核细胞(PBMC)表达的CD54、CD80、HLA-DR及甲状腺过氧化物酶抗体(TPOAb)和甲状腺球蛋白抗体(TGAb)的关系。方法:化学发光法测定正常人、Graves病(GD)和桥本甲状腺炎(HT)患者甲状腺功能、TGAb、TPOAb,放免法测定血肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β),免疫组化法检测PBMC表面CD54、HLA-DR表达,流式细胞仪检测PBMC表面CD80。结果:3组患者外周血IL-1β、TNF-α以及PBMC表达的CD54、CD80、HLA-DR差异有统计学意义(P<0.01)。TPOAb阳性的GD患者IL-1β显著高于TPOAb阴性的GD患者熏经多元回归分析显示CD80与IL-1β密切相关。结论:检测外周血IL-1β可能对评价AITD的免疫状况有一定意义,IL-1β可能通过使CD80表达增加而在AITD尤其是在HT的发病中起重要作用。Objective： To study the relationship between IL-1β and PBMC expressed CD54, CDS0 and HLA-DR, TGAb and TPOAb in autoimmune thyroid disease （AITD）. Methods： The thyroid function, TGAb and TPOAb of the controls, GD and HT patients were measured by chemiluminescence, the serum TNF-α and IL-1β were measured by radioimmunoassay, the expression of CD54 and HLA-DR on PBMC were determined by immunohistochemical methods, and expression of CDS0 was detected by flow cytometry. Results： There were significant differences of cytokine IL-1β, TNF-α and PBMC expressed CD54, C DS0 and HLA- DR in three groups （P 〈 0.01）. The levels of IL- 1β in TPOAb positive GD patients were significantly higher than those in negative patients. CD80 was closely correlated with IL- 1β by multiple linear regression. Conclusion： It＇ s significant to measure serum IL-1β for judging the immune function in AITD patients, and IL-1β may take important role in the immune disorder mechanism of AITD through increasing the expression of CDS0 on PBMC, especially in HT.

关 键 词：自身免疫性甲状腺疾病 白细胞介素-1Β 外周血 单个核细胞 免疫机制 

分 类 号：R581[医药卫生—内分泌]