大鼠心脏缺血再灌损伤与葡萄糖酸镁的保护作用  

Myocardial ischemia-reperfusion injury in rats and the protective effects of magnesium gluconate

作　　者：齐志敏[1] 王倩[2] 张英杰[3] 高东明[4] 

机构地区：[1]锦州医学院药理学教研室,辽宁省锦州市121001 [2]锦州医学院附属第一医院神经科教研室,辽宁省锦州市121001 [3]锦州医学院附属第一医院心血管内科教研室,辽宁省锦州市121001 [4]锦州医学院生理学教研室,辽宁省锦州市121001

出　　处：《中国临床康复》2005年第27期51-53,共3页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁省教育厅科研基金资助项目(A20072106)~~

摘　　要：目的:观察具有改善心肌收缩作用的葡萄糖酸镁对实验性大鼠心肌缺血再灌注损伤的保护作用。方法:实验于2005-02在锦州医学院药理教研室完成。24只SD大鼠随机分为3组,对照组、缺血再灌注组和葡萄糖酸镁组,每组8只。3组大鼠均实施主动脉递引灌流,采用Langendorff方法用KH缓冲液犤(NaCl118.5,NaCO325.0,KH2PO41.2,KCl4.8,MgSO41.2,CaCl22.0,葡萄糖:1.0)mmol/L犦制备离体大鼠心脏缺血再灌注模型,将葡萄糖酸镁(2.4mmol/L)加入灌流液内,停灌时间30min,再灌注时间1h。分别在心脏灌流稳定20min,再灌注60min记录左室收缩压、左室压力变化速率,并收集1min冠状动脉血流量。取左心室标本测总超氧化物歧化酶活性用黄嘌呤氧化酶法,测组织丙二醛含量用硫代巴比妥酸法。结果:SD大鼠共24只均进入结果分析。①再灌注后左室收缩压:葡萄糖酸镁组明显高于缺血再灌注组犤(65.6±3.8,56.1±8.7)mmHg,(t=2.287,P<0.05)犦。②再灌注后左室压力变化速率:葡萄糖酸镁组明显低于对照组犤(1241±202,1889±304)mmHg/s,(t=-3.980,P<0.01)犦。③再灌注后冠状动脉血流量:葡萄糖酸镁组明显高于缺血再灌注组犤(4.5±0.3,3.5±0.5)mL/min,t=5.895,P<0.01犦。④再灌注后左室心肌组织内总超氧化物歧化酶活性:葡萄糖酸镁组明显高于缺血再灌注组犤(37.17±3.42,22.18±4.79)kNU/g,t=4.874,P<0.01犦。⑤再灌注后丙二醛的含量:葡萄糖酸镁组明显低于缺血再灌注组犤(5.25±1.01,10.00±1.13)μmol/g,t=-5.827,P<0.01犦。结论:葡萄糖酸镁对再灌注损伤心肌可改善其收缩功能,并降低心肌组织中的丙二醛含量,从而减少氧自由基的产生,起到抗脂质过氧化而保护心肌的作用。AIM： To observe the protective effect of magnesium gluconate, which has the role of ameliorating myocardial contraction, on myocardial ischemiareperfusion injury in rat.METHODS： The experiment was carried out in the Department of Pharmacology, Jinzhou Medical College in February 2005. Twenty-four SD rats were randomized into three groups with 8 rats in each group： control group, ischemia reperfusion group and magnesium gluconate group. All the rats in the 3 groups were treated with aortic gradual infusion, isolated models of myocardial ischemia reperfusion were established by means of Langendorff with Kreb-Henseleit buffer （NaCl： 118.5 mmol/L, NaCO3 25.0 mmol/L, KHEPO4： 1.2, mmol/L KCl： 4.8 mmol/L, MgSO4： 1.2 mmol/L, CaCl2： 2.0 mmol/L, glucose： 1.0 mmol/L）, 2.4 mmol/L magnesium gluconate was added into the infusion solution, they were treated with ischemia for 30 minutes followed by reperfusion for 60 minutes. Cardiac perfusion was stabilized for 20 minutes, and reperfused for 60 minutes, and the left ventricular systolic pressure and changing rate of left ventricular pressure were recorded, and coronary arterial blood flow in 1 minute was collected. Sampies of left ventricle were taken out to detect the activity of total superoxide dismutase with the method of xanthine oxidase, and the content of malondialdehyde with the method of thiobarbituric acid. RESULTS： All the 24 SD rats were involved in the analysis of results. ① The left ventricular systolic pressure after reperfusion： It was obviously higher in the magnesium gluconate group than in the ischemia reperfusion group [（65.6±3.8）, （56.1±8.7） mm Hg, t=2.287, P 〈 0.05]. ② The changing rate of left ventricular pressure after reperfusion： It was obviously lower in the magnesium gluconate group than in the ischemia reperfusion group[（1241±202）, （1889±304） mm Hg/s, t=-3.980, P 〈 0.01]. ③ The coronary arterial blood flow after reperfusion： It was obviously higher in the magnesium gluconate group than in t

关 键 词：心肌缺血 再灌注损伤 大鼠 

分 类 号：R54[医药卫生—心血管疾病]