维生素C和辅酶Q_(10)对饲喂乙醇兔血浆一氧化氮及其合酶和丙二醛含量的影响  

Effects of vitamin C and coenzyme Q_(10) on plasma contents of nitric oxide nitric oxide synthase and malondialdehyde in rabbits fed with alcohol

作　　者：冯庆芝[1] 

机构地区：[1]菏泽市立医院急诊科,山东省菏泽市274031

出　　处：《中国临床康复》2005年第27期62-63,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察维生素C和辅酶Q10对饲喂乙醇兔血浆一氧化氮及其合酶和丙二醛含量的影响。方法:实验于2004-02在山东省菏泽市立医院中心试验室完成。将30只白色獭兔随机分为3组:正常对照组、饲喂乙醇组和干预治疗组,每组10只。正常对照组给予普通饲料喂养30d,饲喂乙醇组饲喂乙醇30d,干预治疗组饲喂乙醇30d停喂乙醇加喂维生素C、辅酶Q1015d,疗程结束后抽血检测一氧化氮、丙二醛含量及一氧化氮合酶活性。乙醇给药方法为每天清晨饲喂3mL/kg,稀释比例为每3mL乙醇加入10mL蒸馏水。维生素C200mg/kg,辅酶Q100.5mg/kg,同一时间,同一地点,一次灌胃。应用分光光度计检测一氧化氮、丙二醛的含量及一氧化氮合酶的活性。结果:30只白色獭兔均进入结果分析。①一氧化氮合酶活性:正常对照组明显高于干预治疗组犤(391.58±45.57,313.06±411.67)nkat/g,(P<0.05)犦。②一氧化氮含量:饲喂酒精组明显低于干预治疗组犤(36.62±9.50,58.39±10.32)μmol/L,(P<0.05)犦。③丙二醛含量:正常对照组明显低于饲喂酒精组犤(2.68±0.57,4.43±0.91)μmol/L,(P<0.01)犦。结论:乙醇可以降低兔血浆中一氧化氮含量及一氧化氮合酶的活性,提高兔血浆中丙二醛的含量。维生素C和辅酶Q10可提高一氧化氮含量,增强一氧化氮合酶活性,降低丙二醛含量,其作用可能与抑制氧自由基损伤,减轻脂质过氧化反应有关。AIM： To observe the effect.of vitamin C and coenzyme Q10 on the contents of nitric oxide, nitric oxide synthase and malondialdehyde in plasma of rabbits fed with alcohol. METHODS： The experiment was completed in the central laboratory of Department of Emergency, Heze Municipal Hospital in February 2004. Thirty white otter rabbits were randomly divided into 3 groups： normal control group （n=10）, alcohol diet group （n=10） and intervention group （n=10）. The rabbits in the normal control group were fed with common diet, and those in the alcohol diet group and intervention group were fed with alcohol diet for 30 days respectively, and then those in the intervention group were fed with vitamin C and coenzyme Q10 for 15 days. After treatment, blood samples were drawn to detect the contents of nitric oxide and malondialdehyde, and the activity of nitric oxide synthase with spectrophotometer. The alcohol （3 mL/kg） was given every morning, the proportion of dilution was that 3 mL alcohol was added into 10 mL distilled water. Gastric perfusion of 200 mg/kg vitamin C and 0.5 mg/kg coenzyme Q10 was given all at once. RESULTS： All the 30 white otter rabbits were involved in the analysis of results. ① The activity of nitric oxide synthase： It was obviously higher in the normal control group than in the intervention group [（391.58±45.57）,（313.06±11.67） nkat/g, P 〈 0.05]. ② The content of nitric oxide： It was obviously lower in the alcohol diet group than in the intervention group [（36.62±9.50）, （58.39±10.32） μmol/L, P 〈 0.05]. ③ The content of malondialdehyde： It was obviously lower in the normal control group than in the alcohol diet group [（2.68±0.57）, （4.43±0.91） μmol/L, P 〈 0.01]. CONCLUSION： Alcohol can decrease content of nitric oxide and and the activity of nitric oxide synthase, and increase the content of malondialdehyde in the plasma of rabbits. Vitamin C and coenzyme Q,0 can increase the content of nitric oxide, enhance the activity of nitric

关 键 词：乙醇 一氧化氮 丙二醛 干预性研究 

分 类 号：R55[医药卫生—血液循环系统疾病]