疏肝理肺方对应激条件下哮喘大鼠脑海马形态的影响  

Effects of shuganlifei method on the cerebral hippocampus formation in rats with asthma under the condition of stress

作　　者：张宁霞[1] 陈慧娟上海中医药大学基础医学院中医基础理论教研室 倪力强[2] 童瑶[3] 

机构地区：[1]上海中医药大学附属普陀医院康复科,上海市200062 [2]上海医药情报研究所,上海市200063 [3]香港大学中医药学院

出　　处：《中国临床康复》2005年第27期109-111,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨束缚应激哮喘大鼠应用疏肝理肺方药后脑海马形态结构的变化。方法:实验于2002-03/2003-01在上海中医药大学国家重点实验室完成。选择SD雄性大鼠56只,随机分为7组,正常组、哮喘组、应激组、哮喘应激组、地塞米松组、理肺组和疏肝理肺组各8只。正常组和应激组大鼠给予1mL生理盐水腹腔注射,其余各组分别腹腔注射等量抗原液致敏,致敏第8天开始,每日将应激组、哮喘应激组、地塞米松组、理肺组和疏肝理肺组大鼠双后肢用绷带立式固定2h给予束缚应激刺激,共进行3周;同时每日分别给予地塞米松组、理肺组和疏肝理肺组大鼠地塞米松(0.75mg/片)1g/(L·kg)、理肺方(麻黄、桂枝、炙甘草、细辛、干姜、半夏、五味子及白芍均9g)28g/(mL·kg)和疏肝理肺方(在理肺方基础上增加柴胡9g及炙枳实9g)36g/(mL·kg)灌胃。在致敏第15天开始,每日给予哮喘组、哮喘应激组、地塞米松组、理肺组和疏肝理肺组大鼠卵蛋白20g/L雾化吸入3min,共雾化2周。造模及治疗结束后,麻醉大鼠抽取腹主动脉血,测定血浆皮质酮水平应用放射免疫法。抽血后,开颅取大鼠脑,采用光镜及透射电镜检测大鼠脑海马的形态结构。结果:56只大鼠全部进入结果分析,无脱失。①各组大鼠血浆皮质酮水平比较:哮喘组和应激组大鼠的血浆皮质酮水平均较正常组显著升高犤(532.48±115.28),(323.32±100.40),(235.27±46.64)μg/L,(P<0.01,0.05)犦。疏肝理肺方组大鼠较哮喘应激组显著降低犤(309.50±46.52),(569.89±72.32)μg/L(P均<0.01)犦。疏肝理肺方组较理肺组(528.60±130.72)μg/L显著降低(P<0.01)。②各组大鼠光镜下脑海马形态结构的比较:正常组大鼠脑海马锥体细胞排列规则,细胞核圆而大,核仁清晰。哮喘应激组、地塞米松组和理肺组大鼠相似脑海马锥体细胞排列不规则,在CA3区可见大量的锥体细胞核固缩。疏肝理肺组大鼠脑海�AIM： To explore the changes of the formation of cerebral hippocampus in rats with binding stress asthma after treated with shuganlifei method. METHODS： The experiment was conducted in the key laboratory of Shanghai University of Traditional Chinese Medicine between March 2002 and January 2003. Fifty-six SD male rats were selected and assigned randomly into 7 groups： normal group, asthma group, stress group, asthma stress group, desamethasone group, regulating lung group and shuganlifei group with 8 rats in each group. The rats in normal group and stress group were treated with 1 mL saline through intraperitoneal injection. Those in other groups were treated with equivalence antigen fluid induced sensitization respectively through intraperitoneal injection. From the 8^th day after sensitization, the both hindlimb of the rats in stress group, asthma stress group, desamethasone group, regulating lung group and shuganlifei group were fixed by band every day vertically for 2 hours and gave the binding stress stimulation, totally for 3 weeks; Meanwhile, the rats in desamethasone group, regulating lung group and shuganlifei group were given desamethasone （0.75mg/each）1 g/（L ·kg） and regulating lung method （ephedra, cassia twig, asarum, dried ginger, pinellia tuber, Chinese magnoliavine fruit and white peony root, all 9 g） 28 g/（mL ·kg） and shuganlifei method （based on the regulating method, 9 g Chinese thorowax root and 9 g immature bitter orange were added） 36 g/（mL ·kg） for gastric perfusion. From the 15^th day after sensitization, the rats in the asthma group, asthma stress group, desamethasone group, regulating lung group and shuganlifei group were given 20 g/L egg albumin absorbing for 3 minutes, totally for 2 weeks. At the ending of building models and treatment, the blood of abdominal aorta was separated from anesthesia rats. The level of plasm corticosterone was tested by radio-immunity method. After exacting the blood, the brain of rats was obtained by bittern the cranium. The f

关 键 词：疏肝理肺方 应激 哮喘 大鼠 脑海马 

分 类 号：R259.622.5[医药卫生—中西医结合]