植物雌激素α-玉米赤霉醇对去卵巢大鼠凝血及纤溶功能的影响 与补充外源性17β-雌二醇的比较(英文)  

Comparison between phytoestrogen α-zearalanol and supplementary ectogenesis 17 β-estradiol in the effect on coagulation and fibrinolysis in ovariectomized rats

作　　者：王雯[1] 朱广瑾[2] 

机构地区：[1]首都医科大学病理生理学教研室,北京市100054 [2]中国医学科学院中国协和医科大学基础医学研究所病理生理学系,北京市100005

出　　处：《中国临床康复》2005年第27期195-197,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(39730220);北京市优秀人才培养专项经费资助(20042D0501817)~~

摘　　要：背景:天然植物雌激素α-玉米赤霉醇具有与雌激素类似的抗动脉粥样硬化作用,且副作用明显低于雌激素,有一定的应用前景。目的:探讨补充外源性17β-雌二醇及植物雌激素α-玉米赤霉醇对去卵巢大鼠凝血及纤溶功能的影响,并对二者的药物作用进行比较分析。设计:观察对比实验。单位:首都医科大学病理生理学教研室和中国医学科学院中国协和医科大学基础医学研究所病理生理学系。材料:实验于2003-07/09在首都医科大学实验动物科学部完成。选择36只12周龄健康雌性Wistar大鼠,体质量(250±10)g,清洁级,随机分为4组,假手术对照组、去卵巢组、去卵巢+17β-雌二醇组及去卵巢+α-玉米赤霉醇组各9只。方法:假手术对照组开腹而不摘除卵巢。去卵巢组在无菌条件下摘除双侧卵巢复制去卵巢动物模型。去卵巢+17β-雌二醇组和去卵巢+α-玉米赤霉醇组分别在去卵巢14d后肌注17β-雌二醇(1mg/kg)和α-玉米赤霉醇(1mg/kg),3d注射1次,共35d。实验结束后大鼠经颈总动脉采血,分离血浆备用。用凝固法测定血浆凝血酶原时间及活化部分凝血活酶时间。用全自动生化分析仪测定血浆纤维蛋白原水平。用酶联免疫吸附法测定血浆组织因子水平。组织型纤溶酶原激活剂及纤溶酶原激活剂抑制物活性测定均采用发色底物法。同时分离子宫,计算子宫质量与体质量的比值。主要观察指标:①血浆凝血酶原时间、活化部分凝血活酶时间、血浆纤维蛋白原水平、血浆组织因子水平、组织型纤溶酶原激活剂活性及纤溶酶原激活剂抑制物活性。②子宫质量/体质量的比值。结果:36只大鼠全部进入结果分析。①凝血酶原时间变化:去卵巢组显著短于假手术对照组(P<0.01);补充17β-雌二醇和补充α-玉米赤霉醇后长于去卵巢组(P<0.05～0.01)。②纤维蛋白原和组织因子水平变化:去卵巢组显著高于假手术对照BACKGROUND： α-zearalanol, a natural phytoestrogen has the effect of anti- atheroscleresis like the estrogen but with less side effect. Therefore, it has a potential for more application in the future. OBJECTIVE： To investigate the effect of 17β-estradiol （E2） and α-zearalanol （ZAL） on coagulation and fibrinolysis in ovariectomizod rats and to compare and analyze their effects. DESIGN： An observational and controlled experiment. SETTING：Department of Pathophysiology of the Capital University of Medical Sciences and the Department of Pathophsiology of the Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences and Peking Union Medical College. MATERIALS： The experiment was completed at the Science Department of Experiment Animals from July to September 2003. Thirty-six healthy female Wistar rats aged 12-week old , weighing （250±10）g, clean grade were involved. The animals were divided into 4 groups, namely sham-operation control group, ovariectomy （OVX） group, OVX ＋E2 group, OVX＋ZAL group with 9 rats in each group. METHODS： For the rats in the sham-operation control group, operation was performed without removing the ovary. For the rats in the OVX group, ovariectomy was performed and the rat models were made at the sterile condition. 1 mg/kg 17β-estradiol and lmg/kg α-zearalanol were respectively injected intramuscularly into the rats in the OVX ＋E2 group and OVX＋ZAL group 14 days after the operation, once every three days for 35 days altogether. After the administration of 17β-estradiol or α-zearalanol for 5 weeks, rats were killed, and blood was collected through the common carotid artery and plasma was collected from it. The prothrombin time （PT） and the activated partial thromboplastin time （APTT） were determined with coagulation method. The level of fibrinogen （FG） was measured with an automatic biochemistry analyzer. Tissue factor（TF）level was determined with ELISA method, the activity of tissue plasminogen activator（t-PA）and p

关 键 词：卵巢切除术 雌二醇 凝血酶原 纤维蛋白溶酶原 

分 类 号：R55[医药卫生—血液循环系统疾病]