p21,CD_(44S),VEGF脑星形细胞瘤组织表达的意义  

Expression of p21, VEGF and CD_ 44S gene proteins in brain astrocytoma and its clinical significance

作　　者：刘英杰[1] 赵黔鲁[1] 马利杰[1] 王献华[1] 

机构地区：[1]华北煤炭医学院附属医院骨科,河北唐山063000

出　　处：《第四军医大学学报》2005年第15期1417-1420,共4页Journal of the Fourth Military Medical University

摘　　要：目的:通过对星形细胞瘤组织中p21,VEGF,CD44S基因蛋白表达的检测,揭示脑胶质细胞瘤发生、发展、侵袭和播散的机制,为临床诊断、治疗及预后判断提供可靠的理论依据.方法:①实验组:分别在Ⅰ～Ⅳ级星形细胞瘤手术后存档蜡块中随机抽取40例,其中每一级各占10例.对照组:选取10例正常脑组织.②应用免疫组化技术(SP法)检测p21,VEGF,CD44S基因蛋白在星形细胞瘤组织中的表达.③统计学处理:采用SNK两两比较,p21经平方根反正弦变换后再进行成组设计的单因素方差分析,等级相关分析,显著水平为α=0.05.结果:①p21基因蛋白在正常脑组织中表达均阴性,在星形细胞瘤组织中表达增高,阳性率为75%.在Ⅰ～Ⅳ级瘤组织中表达值分别为:1.28±0.07,1.23±0.10,0.94±0.11及0.85±0.12,说明p21表达随肿瘤恶性度的增高而降低,在高分化与低分化星形细胞瘤之间存在明显的差异性(P<0.01).②VEGF基因蛋白在正常脑组织中表达均阴性,在星形细胞瘤组织中表达增高,其静灰度值在Ⅰ～Ⅳ级瘤组织中分别为108±7,105±8,96±11及95±9,说明VEGF随肿瘤恶性度的增高而增加,在正常脑组织、低分化及高分化星形细胞瘤瘤组织间具有明显的差异性(P<0.01).③CD44S基因蛋白在正常脑组织中表达均阴性,在星形细胞瘤组织中表达增高,阳性率为100%.其静灰度值在Ⅰ～Ⅳ级中分别为120±11,113±11,99±11及91±14,说明CD44S表达与病理学分级临床分期、颅内侵袭有明显相关性(P<0.01).结论:①脑胶质瘤的形成是多因素、多途径共同作用的结果.②p21,VEGF,CD44S可作为判断脑胶质细胞瘤恶性程度及评估预后的分子标志.AIM： To identify the protein expression of p21,vascular growth factor （VEGF） and CD44s in the astrocytoma to find the mechanism of tumorigenesis, growth, invasion and diffusion. METHODS： ①The experimental group consisted of forty paraffin-embedded samples of brain astrocytoma with different pathologic types while the control group consisted of ten normal brain tissues.②The expressions of p21, VEGF and CD,s were detected by immuno-histochemistry technology. ③ Significance was set at α =0.05 and Student-Newnan-Keuls test for variables was performed for statistic evaluation. RESULTS： ①p21 protein expression was negative in all the ten normal brain tissues and its positive expression rate was 75% in brain astrocytoma tissues. But the rates were inversely correlated with increased histological grade and they were 1. 28 ± 0. 07,1.23 ±0. 10, 0.94 ±0. 11 and 0.85±0. 12 respectively inbrain astrocytoma of grade Ⅰ , Ⅱ, Ⅲ and Ⅳ. The difference between the lower and higher grades was significant（P〈0.01）. ②VEGF expression of protein was negative in all the ten normal brain tissues, but strongly positive in brain astrocytoma and was correlated with increased histological grade and they were 108 ± 7,105 ± 8, 96 ± 11 and 95 ± 9 respectively in the brain astrocytoma of grade Ⅰ, Ⅱ ⅢandⅣ. The difference between lower and higher grade was significant （P〈0.01）. ③CD44S expression of protein was negative in all the ten normal brain tissues, but strongly positive in brain astrocytoma and was correlated with increased histological grade and they were 120 ± 11, 113 ± 11, 99 ±11 and 91 ± 14 respectively in the brain astrocytoma of grade Ⅰ , Ⅱ, Ⅲ and Ⅳ. The difference between normal, lower and higher of grade was significant （ P 〈0.01 ）, CONCLUSION： The brain astrocytoma results from the interaction of many factors and pathways. ②The proteins of p21,VEGF and CD44s can be taken as molecular markers for the evaluation of malignant grade and the prognosis of brai

关 键 词：脑 星形细胞瘤 P21基因 CD44S黏附分子 

分 类 号：R631[医药卫生—外科学]