多聚次黄苷酸-胞苷酸诱发性小鼠流产模型CD200^+CK7^+滋养层细胞分析  

Murine CD200^+CK7^+ trophoblasts in poly (I∶C)-induced embryo resorption model

作　　者：林羿[1] 曾耀英[1] 狄静芳[1] 曾山[1] 肇静娴[1] 王通[1] 曾祥凤[1] 冯铮[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《中国病理生理杂志》2005年第8期1498-1502,共5页Chinese Journal of Pathophysiology

基　　金：暨南大学引进优秀人才科研启动基金(No.51204067);广东省自然科学基金资助项目(No.04300213);中国博士后科学基金资助项目(No.2002032244)

摘　　要：目的:在多聚次黄苷酸-胞苷酸[poly(I∶C)]诱发性流产小鼠模型中研究CD200+CK7+细胞与胚胎吸收的关系。方法:采用Balb/c×C57BL/6和Balb/c×Balb/c小鼠,在孕早期注射poly(I∶C)以建立诱发性流产模型,并采用流式细胞术检测胎盘CD200+CK7+/CK7+细胞百分率和CD200+CK7+细胞的绝对数,采用免疫组化法对CD200+细胞在母-胎界面的分布情况进行定位检测。结果:在每例检测104个细胞的情况下,poly(I∶C)处理组上述细胞百分率和绝对数均显著低于对照组(Balb/c×C57BL/6小鼠:6·3%±6·2%vs36·1%±9·3%,P<0·01;140±111vs1941±809,P<0·01。Balb/c×Balb/c小鼠:8·5%±4·8%vs26·1%±8·0%,P<0·01;701±499vs1886±1112,P<0·05)。与此相应,poly(I∶C)处理组孕13·5d的胚胎吸收率也显著增高(Balb/c×C57BL/6小鼠:从9·1%升至37·0%,P<0·01;Balb/c×Balb/c小鼠:从5·8%升至29·0%,P<0·01)。免疫组化法检测发现,这些CD200+细胞主要分布在胎盘和子宫交界处的胎盘组织中。结论:在母-胎界面CK7+细胞群中CD200分子适当水平的表达,对于维持小鼠妊娠免疫耐受过程可能具有重要意义。AIM： To investigate the relationship between CD200^＋ CK7^＋trophoblasts and the resorption of embryos in a poly （I：C） - induced abortion model. METHODS： The status of CD200 expression was investigated in Balb/c × C57BL/6 and Balb/c x Balb/c mice as induced model of embryo - resorption by an i.p. injection of poly （ I ： C）. CD200 expression on CK7^＋ cells from placentas was detected with flow cytometry. CD200^＋ cells in placenta were observed with immunocytochemical staining.RESULTS： Both the percentage and absolute number of CD200^＋CK7^＋ cells were dramatically decreased by injection of poly （I：C） in Balb/c x C57BL/6 （6.3% ±6.2% vs 36.1% ±9.3%, P〈0.01; and 140± 111 vs 1 941 ±809, P〈0.01）, as well as in Balb/c × Balb/c mice （8.5% ±4.8% vs 26.1% ± 8.0%, P〈0.01; and 701 ±499 vs 1 886± 1 112, P〈0.05）, with PBS- treated mice as controls. In addition, the lower cell number was statistically correlated with the elevated embryo- resorption detected on day 13.5： from 9.1% to 37.0% in Balb/c ×C57BL/6 mice and from 5.8% to 29.0% in Balb/c x Balb/c mice, respectively （P 〈 0.01 ）. In immunocytochemical analysis, CD200^＋ cells mainly scattered in placenta tissue adjacent to the interface of placenta and uterine. CONCLUSION： Sufficient expression of CD200 on CK7^＋ cells at the feto- maternal interface may be necessary for the maintenance of embryos.

关 键 词：流产 滋养层 角蛋白 

分 类 号：R392.32[医药卫生—免疫学]